Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   MSA_RS01125 Genome accession   NC_021486
Coordinates   191248..191703 (+) Length   151 a.a.
NCBI ID   WP_016480233.1    Uniprot ID   -
Organism   Streptococcus agalactiae ILRI005     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 186248..196703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MSA_RS01110 (MSA_2330) - 188806..189171 (+) 366 WP_016480230.1 DUF1033 family protein -
  MSA_RS01115 (MSA_2340) comGA 189343..190314 (+) 972 WP_016480231.1 competence type IV pilus ATPase ComGA Machinery gene
  MSA_RS01120 (MSA_2350) comGB 190208..191266 (+) 1059 Protein_165 competence type IV pilus assembly protein ComGB -
  MSA_RS01125 (MSA_2360) comGF 191248..191703 (+) 456 WP_016480233.1 competence type IV pilus minor pilin ComGF Machinery gene
  MSA_RS01130 (MSA_2370) comGG 191681..192052 (+) 372 WP_016480234.1 competence type IV pilus minor pilin ComGG Machinery gene
  MSA_RS01135 (MSA_2380) comGH 192167..193141 (+) 975 WP_001008571.1 class I SAM-dependent methyltransferase Machinery gene
  MSA_RS01140 (MSA_2390) - 193173..194366 (+) 1194 WP_000047534.1 acetate kinase -
  MSA_RS01145 (MSA_2400) - 194494..194701 (+) 208 Protein_170 helix-turn-helix transcriptional regulator -
  MSA_RS11940 (MSA_2410) - 194760..194891 (+) 132 WP_079269272.1 hypothetical protein -
  MSA_RS01150 - 194932..195386 (+) 455 Protein_172 hypothetical protein -
  MSA_RS01155 (MSA_2420) - 195455..196120 (+) 666 WP_000008113.1 type II CAAX endopeptidase family protein -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 17534.39 Da        Isoelectric Point: 9.4384

>NTDB_id=49042 MSA_RS01125 WP_016480233.1 191248..191703(+) (comGF) [Streptococcus agalactiae ILRI005]
MENLLLKCKDKKVKAFTLLECLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNK
LYLRKQDKIVTFGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKHVFYFKDGLKRTFYYDFKEET

Nucleotide


Download         Length: 456 bp        

>NTDB_id=49042 MSA_RS01125 WP_016480233.1 191248..191703(+) (comGF) [Streptococcus agalactiae ILRI005]
ATGGAAAATTTATTGTTAAAATGTAAGGATAAGAAGGTTAAAGCATTTACACTTTTAGAATGTTTGGTAGCATTGGTTAC
AATTACAGGAGCTTTACTAGTTTATCAAGGACTGACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTTCCAGTC
AGTCTGAATGGGTGTTATTAACTCAGCAACTAAATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAGAACAAA
CTTTATTTACGTAAGCAAGATAAGATTGTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTATGATGG
TCGAGGTTATCAACCAATGGTTTATGGGTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACATGTTTTTT
ATTTTAAGGACGGGTTAAAAAGGACATTTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Streptococcus mutans UA159

47.857

92.715

0.444
  comGF Streptococcus mutans UA140

47.857

92.715

0.444
  comGF/cglF Streptococcus pneumoniae Rx1

42

99.338

0.417
  comGF/cglF Streptococcus pneumoniae D39

42

99.338

0.417
  comGF/cglF Streptococcus pneumoniae R6

42

99.338

0.417
  comGF/cglF Streptococcus pneumoniae TIGR4

42

99.338

0.417
  comGF/cglF Streptococcus mitis SK321

44.203

91.391

0.404
  comGF/cglF Streptococcus mitis NCTC 12261

43.478

91.391

0.397
  comGF Lactococcus lactis subsp. cremoris KW2

40.58

91.391

0.371

Multiple sequence alignment