Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   KFU67_RS02205 Genome accession   NZ_CP074031
Coordinates   475886..477124 (+) Length   412 a.a.
NCBI ID   WP_000816003.1    Uniprot ID   -
Organism   Escherichia coli strain PK12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470886..482124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU67_RS02180 (KFU67_02135) mrcA 471166..473718 (-) 2553 WP_001361658.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  KFU67_RS02185 (KFU67_02140) hofM 473838..474617 (+) 780 WP_000874542.1 DNA utilization protein HofM -
  KFU67_RS02190 (KFU67_02145) hofN 474617..475156 (+) 540 WP_001069327.1 DNA utilization protein HofN -
  KFU67_RS02195 (KFU67_02150) hofO 475140..475580 (+) 441 WP_000367363.1 DNA utilization protein HofO -
  KFU67_RS02200 (KFU67_02155) hofP 475570..475974 (+) 405 WP_001361657.1 DNA utilization protein HofP -
  KFU67_RS02205 (KFU67_02160) comE 475886..477124 (+) 1239 WP_000816003.1 DNA uptake porin HofQ Machinery gene
  KFU67_RS02210 (KFU67_02165) aroK 477525..478046 (+) 522 WP_000818618.1 shikimate kinase AroK -
  KFU67_RS02215 (KFU67_02170) aroB 478103..479191 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  KFU67_RS02220 (KFU67_02175) damX 479283..480569 (+) 1287 WP_000343177.1 cell division protein DamX -
  KFU67_RS02225 (KFU67_02180) dam 480676..481512 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44751.24 Da        Isoelectric Point: 5.9488

>NTDB_id=490179 KFU67_RS02205 WP_000816003.1 475886..477124(+) (comE) [Escherichia coli strain PK12]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=490179 KFU67_RS02205 WP_000816003.1 475886..477124(+) (comE) [Escherichia coli strain PK12]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAATATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCCCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCAGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAAGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362