Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGH   Type   Machinery gene
Locus tag   BSA_RS01115 Genome accession   NC_021485
Coordinates   190858..191832 (+) Length   324 a.a.
NCBI ID   WP_001008574.1    Uniprot ID   Q8E232
Organism   Streptococcus agalactiae 09mas018883     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 185858..196832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSA_RS01075 (BSA_2200) - 186548..186913 (+) 366 WP_000285373.1 DUF1033 family protein -
  BSA_RS01080 (BSA_2210) comGA 187086..188057 (+) 972 WP_000250805.1 competence type IV pilus ATPase ComGA Machinery gene
  BSA_RS01085 (BSA_2220) comGB 187951..188994 (+) 1044 WP_223295889.1 competence type IV pilus assembly protein ComGB Machinery gene
  BSA_RS01090 (BSA_2230) comGC/cglC 188991..189320 (+) 330 WP_000793380.1 competence type IV pilus major pilin ComGC Machinery gene
  BSA_RS01095 (BSA_2240) comGD 189295..189708 (+) 414 WP_000244261.1 competence type IV pilus minor pilin ComGD Machinery gene
  BSA_RS01100 (BSA_2250) comGE 189680..189979 (+) 300 WP_000234804.1 competence type IV pilus minor pilin ComGE Machinery gene
  BSA_RS01105 (BSA_2260) comGF 189933..190394 (+) 462 WP_001878820.1 competence type IV pilus minor pilin ComGF Machinery gene
  BSA_RS01110 (BSA_2270) comGG 190372..190743 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG Machinery gene
  BSA_RS01115 (BSA_2280) comGH 190858..191832 (+) 975 WP_001008574.1 N-6 DNA methylase Machinery gene
  BSA_RS01120 (BSA_2290) - 191864..193057 (+) 1194 WP_000047534.1 acetate kinase -
  BSA_RS01125 (BSA_2300) - 193209..193415 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  BSA_RS11575 (BSA_2310) - 193474..193611 (+) 138 WP_001865900.1 hypothetical protein -
  BSA_RS01130 (BSA_2320) - 193652..194107 (+) 456 WP_000905673.1 hypothetical protein -
  BSA_RS01135 (BSA_2330) - 194176..194841 (+) 666 WP_000008113.1 type II CAAX endopeptidase family protein -
  BSA_RS01140 (BSA_2340) proC 194862..195632 (-) 771 WP_001865901.1 pyrroline-5-carboxylate reductase -
  BSA_RS01145 (BSA_2350) pepA 195702..196769 (-) 1068 WP_001281323.1 glutamyl aminopeptidase -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 37046.02 Da        Isoelectric Point: 4.5224

>NTDB_id=48940 BSA_RS01115 WP_001008574.1 190858..191832(+) (comGH) [Streptococcus agalactiae 09mas018883]
MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSCDLDMVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQT
EQLQANHQFTPDSIGFILLFLLEELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
AQFIQEDAVRPQILKESDVIISDLPVGYYPNDGIAKRYAVSSSKEHTYAHHLLMEQSLKYLKKDGIAIFLAPENLLTSPQ
SDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFVLKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSK
NMIK

Nucleotide


Download         Length: 975 bp        

>NTDB_id=48940 BSA_RS01115 WP_001008574.1 190858..191832(+) (comGH) [Streptococcus agalactiae 09mas018883]
ATGAATTTTGAAAAAATTGAGACAGCCTATGAGCTGATTTTAGAAAATATCCAAACGATTGAGAACCAATTAAAAACTCA
TATTTATGATGCCTTAATTGAACAGAACTCTTATTACCTTGGTTCAAGTTGTGATTTAGATATGGTTGTGGTGAATAACC
AAAAATTACGTCAACTTGACTTAAGTCAAGAAGAATGGCGTCGCACTTTCCAGTTCATTTTTATCAAATCTGCGCAAACA
GAGCAATTACAAGCTAATCATCAGTTTACGCCAGATAGTATTGGTTTTATCTTGTTATTTCTTTTGGAAGAATTAACGAG
TCAAGAGACAGTGGATGTCTTGGAAATTGGAAGTGGAACTGGGAATTTAGCTCAGACTCTCCTCAATAACAGCTCGAAAG
AGTTAAATTATATGGGCATTGAAGTTGATGATCTTTTGATTGATCTATCAGCAAGCATTGCTGAAATTATAGGTTCTAGT
GCCCAATTTATCCAAGAGGATGCTGTTAGACCACAAATTTTGAAAGAAAGCGATGTAATCATTAGTGATTTACCAGTTGG
CTATTATCCTAATGATGGTATTGCTAAACGATATGCTGTATCAAGTTCTAAAGAGCACACCTATGCTCACCATCTATTGA
TGGAGCAATCTCTTAAATATTTGAAAAAAGATGGAATCGCTATATTTTTAGCACCCGAAAACCTTTTAACAAGTCCACAA
AGTGATTTGCTGAAGGAGTGGTTAAAAGGATATGCAGATGTCATTGCCGTTTTAACTCTACCAGAAACTATTTTTGGAAG
TCGTCAAAATGCGAAATCTATATTTGTTCTCAAGAAGCAAGCAGAACAAAAACCAGAAACCTTTGTATATCCGCTGACAG
ATTTGCAAAATCGTGAGAATATGGCAAACTTCATTGAAAATTTTCAAAAATGGAGCAGAGAAAATAGTCATTACTCAAAA
AATATGATAAAATAG

Domains

Predicted by InterProScan.

N-6 DNA Methylase (N6_Mtase)

(70-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8E232

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGH Streptococcus mutans UA159

67.302

97.222

0.654
  comGH Streptococcus mutans UA140

67.302

97.222

0.654

Multiple sequence alignment