Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   KFU82_RS01850 Genome accession   NZ_CP073968
Coordinates   404383..405621 (+) Length   412 a.a.
NCBI ID   WP_000815986.1    Uniprot ID   -
Organism   Escherichia coli strain MB30     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 399383..410621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU82_RS01825 (KFU82_01780) mrcA 399663..402215 (-) 2553 WP_001300707.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  KFU82_RS01830 (KFU82_01785) hofM 402335..403114 (+) 780 WP_001295166.1 DNA utilization protein HofM -
  KFU82_RS01835 (KFU82_01790) hofN 403114..403653 (+) 540 WP_058905867.1 DNA utilization protein HofN -
  KFU82_RS01840 (KFU82_01795) hofO 403637..404077 (+) 441 WP_001055760.1 DNA utilization protein HofO -
  KFU82_RS01845 (KFU82_01800) hofP 404067..404471 (+) 405 WP_001264141.1 DNA utilization protein HofP -
  KFU82_RS01850 (KFU82_01805) comE 404383..405621 (+) 1239 WP_000815986.1 DNA uptake porin HofQ Machinery gene
  KFU82_RS01855 (KFU82_01810) aroK 406022..406543 (+) 522 WP_000818618.1 shikimate kinase AroK -
  KFU82_RS01860 (KFU82_01815) aroB 406600..407688 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  KFU82_RS01865 (KFU82_01820) damX 407780..409066 (+) 1287 WP_000343205.1 cell division protein DamX -
  KFU82_RS01870 (KFU82_01825) dam 409173..410009 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44743.26 Da        Isoelectric Point: 6.3183

>NTDB_id=488985 KFU82_RS01850 WP_000815986.1 404383..405621(+) (comE) [Escherichia coli strain MB30]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSNE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=488985 KFU82_RS01850 WP_000815986.1 404383..405621(+) (comE) [Escherichia coli strain MB30]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGCCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAATGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.327

100

0.393
  comE Haemophilus influenzae 86-028NP

37.097

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.005

100

0.388
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362