Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   KEF49_RS12745 Genome accession   NZ_CP073635
Coordinates   2532571..2533575 (-) Length   334 a.a.
NCBI ID   WP_065981977.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Ba13     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2527571..2538575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEF49_RS12715 (KEF49_12715) - 2528598..2529254 (-) 657 WP_065981974.1 DUF421 domain-containing protein -
  KEF49_RS12720 (KEF49_12720) - 2529421..2529807 (+) 387 WP_013353039.1 TIGR04086 family membrane protein -
  KEF49_RS12725 (KEF49_12725) yajC 2529859..2530119 (-) 261 WP_003152695.1 preprotein translocase subunit YajC -
  KEF49_RS12730 (KEF49_12730) tgt 2530151..2531296 (-) 1146 WP_014472142.1 tRNA guanosine(34) transglycosylase Tgt -
  KEF49_RS12735 (KEF49_12735) queA 2531324..2532352 (-) 1029 WP_065522675.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  KEF49_RS12740 (KEF49_12740) - 2532378..2532578 (-) 201 WP_065981976.1 DUF2905 domain-containing protein -
  KEF49_RS12745 (KEF49_12745) ruvB 2532571..2533575 (-) 1005 WP_065981977.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  KEF49_RS12750 (KEF49_12750) ruvA 2533586..2534191 (-) 606 WP_003152683.1 Holliday junction branch migration protein RuvA Machinery gene
  KEF49_RS12755 (KEF49_12755) - 2534326..2534802 (-) 477 WP_038463270.1 intercompartmental signaling factor BofC -
  KEF49_RS12760 (KEF49_12760) - 2535212..2535805 (-) 594 WP_065981978.1 YhcN/YlaJ family sporulation lipoprotein -
  KEF49_RS12765 (KEF49_12765) safA 2535953..2537113 (-) 1161 WP_065981979.1 spore coat assembly protein SafA -
  KEF49_RS12770 (KEF49_12770) nadA 2537240..2538343 (-) 1104 WP_115997698.1 quinolinate synthase NadA -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37200.52 Da        Isoelectric Point: 4.7246

>NTDB_id=487658 KEF49_RS12745 WP_065981977.1 2532571..2533575(-) (ruvB) [Bacillus amyloliquefaciens strain Ba13]
MDERLVSSEADSHESIIEQSLRPQNLAQYIGQQKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
MRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLNRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVLSRLEYYTKEELTDIVSRTAEVFEVEIEEAPALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEEIAHDALERLQVDALGLDHIDHKLLMGMIEKFSGGPVGLDTISATIGEEPHTIEDVYEPYLLQIGFLQRTPRGRVVTP
AVYEHFRLEAPACD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=487658 KEF49_RS12745 WP_065981977.1 2532571..2533575(-) (ruvB) [Bacillus amyloliquefaciens strain Ba13]
ATGGATGAACGGCTCGTTTCAAGTGAAGCAGACAGCCATGAATCAATAATCGAGCAAAGCCTCAGGCCGCAGAACCTCGC
CCAGTACATCGGGCAGCAGAAGGTAAAAGAAAATCTGCGCGTGTTTATCGATGCGGCGAAAATGAGGCAGGAAACGCTTG
ACCACGTTCTTTTATACGGGCCTCCGGGTCTCGGAAAAACAACGCTTGCGTCTATCGTGGCCAATGAGATGGGTGTGGAG
ATGCGGACGACGTCAGGACCCGCTATCGAAAGACCGGGAGACTTGGCAGCCATTCTGACGGCGCTCGAACCCGGGGATGT
GCTGTTTATAGACGAAATTCACAGGCTGAACCGGTCAATCGAAGAAGTGCTGTACCCGGCGATGGAGGATTTCTGCCTTG
ATATCGTCATTGGTAAAGGCCCGTCCGCCCGCTCCGTCCGCCTTGACCTTCCGCCTTTTACGCTGGTAGGGGCGACGACC
AGGGTCGGCTTATTGACCGCGCCGCTCAGAGACCGGTTCGGGGTGCTGTCCCGCCTTGAATACTATACGAAGGAAGAACT
GACAGATATCGTGTCAAGAACGGCTGAAGTATTCGAAGTTGAAATCGAAGAAGCCCCGGCTTTGGAAATCGCCAGACGGT
CAAGGGGAACGCCGCGGGTTGCAAACCGGCTTCTCAGAAGGGTGAGAGATTTTGCCCAGGTGCTCGGCGACAGCCGCATC
ACGGAAGAAATCGCTCACGACGCGCTCGAACGTCTTCAAGTCGACGCGCTCGGCCTTGACCATATTGACCATAAGCTGCT
GATGGGCATGATTGAAAAATTCAGCGGCGGTCCCGTCGGCCTTGATACGATTTCCGCGACAATCGGAGAAGAACCGCATA
CAATAGAAGATGTGTACGAACCGTATCTTCTTCAGATCGGATTTCTCCAAAGGACGCCGAGAGGACGGGTCGTGACCCCG
GCTGTGTATGAGCATTTTCGATTGGAGGCACCCGCCTGTGACTGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

91.617

100

0.916
  ruvB Streptococcus pneumoniae TIGR4

60.736

97.605

0.593
  ruvB Streptococcus pneumoniae R6

60.736

97.605

0.593
  ruvB Streptococcus pneumoniae D39

60.736

97.605

0.593
  ruvB Synechocystis sp. PCC 6803

58.359

98.503

0.575
  ruvB Helicobacter pylori 26695

52.439

98.204

0.515