Detailed information NTG0487659

Bioinformatically predicted

Overview

• Name: ruvA
• Type: Machinery gene
• Locus tag: KEF49_RS12750
• Genome accession: NZ_CP073635
• Coordinates: 2533586..2534191 (-)
• Length: 201 a.a.
• NCBI ID: WP_003152683.1
• Uniprot ID: A7Z769
• Organism: Bacillus amyloliquefaciens strain Ba13
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2528586..2539191

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KEF49_RS12715 (KEF49_12715)	-	2528598..2529254 (-)	657	WP_065981974.1	DUF421 domain-containing protein	-
KEF49_RS12720 (KEF49_12720)	-	2529421..2529807 (+)	387	WP_013353039.1	TIGR04086 family membrane protein	-
KEF49_RS12725 (KEF49_12725)	yajC	2529859..2530119 (-)	261	WP_003152695.1	preprotein translocase subunit YajC	-
KEF49_RS12730 (KEF49_12730)	tgt	2530151..2531296 (-)	1146	WP_014472142.1	tRNA guanosine(34) transglycosylase Tgt	-
KEF49_RS12735 (KEF49_12735)	queA	2531324..2532352 (-)	1029	WP_065522675.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
KEF49_RS12740 (KEF49_12740)	-	2532378..2532578 (-)	201	WP_065981976.1	DUF2905 domain-containing protein	-
KEF49_RS12745 (KEF49_12745)	ruvB	2532571..2533575 (-)	1005	WP_065981977.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
KEF49_RS12750 (KEF49_12750)	ruvA	2533586..2534191 (-)	606	WP_003152683.1	Holliday junction branch migration protein RuvA	Machinery gene
KEF49_RS12755 (KEF49_12755)	-	2534326..2534802 (-)	477	WP_038463270.1	intercompartmental signaling factor BofC	-
KEF49_RS12760 (KEF49_12760)	-	2535212..2535805 (-)	594	WP_065981978.1	YhcN/YlaJ family sporulation lipoprotein	-
KEF49_RS12765 (KEF49_12765)	safA	2535953..2537113 (-)	1161	WP_065981979.1	spore coat assembly protein SafA	-
KEF49_RS12770 (KEF49_12770)	nadA	2537240..2538343 (-)	1104	WP_115997698.1	quinolinate synthase NadA	-
KEF49_RS12775 (KEF49_12775)	nadC	2538343..2539191 (-)	849	WP_115997699.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 201 a.a.
• Molecular weight: 22414.85 Da
• Isoelectric Point: 5.1597

>NTDB_id=487659 KEF49_RS12750 WP_003152683.1 2533586..2534191(-) (ruvA) [Bacillus amyloliquefaciens strain Ba13]
MIEFVKGTIDYVSPQYIVIENGGIGYQVFTPNPFIYKVSNQETIFTYHHIKEDAFSLYGFSTREEKALFTKLLNVTGIGP
KGALAILGSGDPGAVIEAIEQEDEAFLVKFPGVGKKTARQIILDLKGKLADVVPEMIDNLFNHEARIEKQEAETALDEAL
EALRVLGYAEKEIKKVLPHLKEETALSTDQYVKKALQKLLK

Nucleotide

• Length: 606 bp

>NTDB_id=487659 KEF49_RS12750 WP_003152683.1 2533586..2534191(-) (ruvA) [Bacillus amyloliquefaciens strain Ba13]
GTGATTGAATTCGTGAAGGGGACGATTGATTATGTATCTCCCCAATACATCGTGATTGAAAACGGCGGGATCGGCTATCA
GGTCTTCACTCCGAATCCGTTTATTTATAAGGTAAGCAATCAAGAGACCATTTTTACGTATCATCATATAAAAGAAGACG
CCTTTTCTTTGTACGGCTTTTCAACCCGGGAAGAAAAAGCGCTTTTCACAAAGCTGCTGAATGTCACCGGCATCGGTCCG
AAAGGGGCGCTCGCCATTCTCGGTTCCGGTGATCCGGGAGCCGTCATCGAAGCGATCGAACAGGAAGATGAAGCCTTTTT
GGTGAAATTTCCTGGTGTGGGCAAAAAAACGGCACGGCAGATTATTCTTGATTTGAAAGGCAAACTGGCCGATGTCGTGC
CTGAAATGATTGACAACCTGTTCAATCATGAAGCGCGCATTGAAAAACAAGAGGCTGAAACGGCGCTTGATGAGGCGCTT
GAAGCGCTCAGGGTTCTCGGCTACGCCGAAAAAGAAATTAAAAAAGTGCTTCCTCACTTAAAAGAGGAAACGGCGCTTTC
AACAGACCAATATGTGAAAAAAGCATTGCAAAAATTATTAAAGTAA

Domains

Predicted by InterProScan.

• RuvA, C-terminal domain (RuvA_C) (155-199)

• RuvA N terminal domain (RuvA_N) (1-59)

• Helix-hairpin-helix domain (HHH_5) (69-126)

3D structure

• AlphaFold DB: A7Z769

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvA	Bacillus subtilis subsp. subtilis str. 168	92.537	100	0.925
ruvA	Streptococcus pneumoniae R6	39.806	100	0.408
ruvA	Streptococcus pneumoniae D39	39.806	100	0.408
ruvA	Streptococcus pneumoniae TIGR4	39.806	100	0.408