Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   KEW59_RS14790 Genome accession   NZ_CP073621
Coordinates   3127219..3128712 (+) Length   497 a.a.
NCBI ID   WP_001596191.1    Uniprot ID   -
Organism   Escherichia coli strain CE2050     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3122219..3133712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEW59_RS14770 (KEW59_14655) pilD 3122867..3123676 (+) 810 WP_001596192.1 prepilin peptidase PppA Machinery gene
  KEW59_RS14775 (KEW59_14660) gspS2 3123742..3124152 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  KEW59_RS14780 (KEW59_14665) gspC 3124170..3125129 (+) 960 WP_024190717.1 type II secretion system protein GspC -
  KEW59_RS14785 (KEW59_14670) gspD 3125159..3127219 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  KEW59_RS14790 (KEW59_14675) pilB/pilB1 3127219..3128712 (+) 1494 WP_001596191.1 type II secretion system ATPase GspE Machinery gene
  KEW59_RS14795 (KEW59_14680) gspF 3128712..3129935 (+) 1224 WP_001596190.1 type II secretion system inner membrane protein GspF -
  KEW59_RS14800 (KEW59_14685) gspG 3129952..3130407 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  KEW59_RS14805 (KEW59_14690) gspH 3130411..3130974 (+) 564 WP_001115129.1 type II secretion system minor pseudopilin GspH -
  KEW59_RS14810 (KEW59_14695) gspI 3130971..3131342 (+) 372 WP_000820072.1 type II secretion system minor pseudopilin GspI -
  KEW59_RS14815 (KEW59_14700) gspJ 3131339..3131944 (+) 606 WP_001250454.1 type II secretion system minor pseudopilin GspJ -
  KEW59_RS14820 (KEW59_14705) gspK 3131941..3132918 (+) 978 WP_001596189.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55169.27 Da        Isoelectric Point: 5.9800

>NTDB_id=487446 KEW59_RS14790 WP_001596191.1 3127219..3128712(+) (pilB/pilB1) [Escherichia coli strain CE2050]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYIRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=487446 KEW59_RS14790 WP_001596191.1 3127219..3128712(+) (pilB/pilB1) [Escherichia coli strain CE2050]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGACTGAATGCCGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCCGAGGACTTCTTTACCCTCGCAGAAGAGCTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATCGACGTGCGTGTCTCCACCATGCCATCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCACGAACTGACC
GCGCAGTTGCGCCAGCTACTGCACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTTCAGGCGTCGCTCACTGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCATGCCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATATCCGGCAACACTCTGCGGGCATCCGCAGCAATGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.059

85.513

0.402
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.867

80.282

0.376
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilB Acinetobacter baylyi ADP1

46.41

78.471

0.364
  pilF Thermus thermophilus HB27

45

80.483

0.362