Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   IHQ43_RS02145 Genome accession   NZ_CP062253
Coordinates   486482..487546 (+) Length   354 a.a.
NCBI ID   WP_007952389.1    Uniprot ID   J2PGW6
Organism   Pseudomonas gozinkensis strain IzPS32d     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481482..492546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHQ43_RS02135 - 482284..483552 (+) 1269 WP_007952395.1 malic enzyme-like NAD(P)-binding protein -
  IHQ43_RS02140 - 483817..486264 (-) 2448 WP_192563212.1 penicillin-binding protein 1A -
  IHQ43_RS02145 comM 486482..487546 (+) 1065 WP_007952389.1 pilus assembly protein PilM Machinery gene
  IHQ43_RS02150 - 487546..488112 (+) 567 WP_192563213.1 PilN domain-containing protein -
  IHQ43_RS02155 pilO 488109..488732 (+) 624 WP_192563214.1 type 4a pilus biogenesis protein PilO -
  IHQ43_RS02160 - 488729..489253 (+) 525 WP_192563215.1 pilus assembly protein PilP -
  IHQ43_RS02165 pilQ 489268..491346 (+) 2079 WP_192563216.1 type IV pilus secretin PilQ Machinery gene
  IHQ43_RS02170 aroK 491351..491869 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38097.60 Da        Isoelectric Point: 4.6857

>NTDB_id=487381 IHQ43_RS02145 WP_007952389.1 486482..487546(+) (comM) [Pseudomonas gozinkensis strain IzPS32d]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPASAVVEKNIAELEGVGQALSRVLVKARTGLKTVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQDRLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEERLNTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=487381 IHQ43_RS02145 WP_007952389.1 486482..487546(+) (comM) [Pseudomonas gozinkensis strain IzPS32d]
GTGCTAGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGCTCCACTTCGGTGAAGCTGCTGGAGCT
GAGCCGCCAAGGTGAGCGCTACCGGGTCGAGGCGTACGCGGTGGAGCCGTTGCCGGCGAGTGCCGTGGTCGAAAAGAATA
TCGCCGAGCTCGAAGGCGTCGGCCAGGCCCTGTCCCGAGTGCTGGTCAAGGCGCGCACCGGGTTGAAAACCGTGGCGGTG
GCAGTGGCCGGCTCGGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAGCTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGACGAGGTCGCCATCGACTTTGAAGTCCAGGGCGTTTCGCCGC
GCAACCCCGAGCGGGTCAACGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAGGCGGCACTCGCCCTC
GCCGGGCTGACCGCGCGAGTGGTGGATGTCGAAGCCTATGCACTGGAGCGCTCGTTCGGCCTGCTGGCCACCCAACTCGC
CGCGTCCCAGGATCGGCTGACAGTGGCCGTGGTCGATATCGGCGCCACCATGACTACCCTCAGCGTCCTGCACAACGGCC
GGATCATCTACACCCGCGAACAACTGTTCGGCGGTCGACAACTGACCGAGGAAATCCAGCGCCGTTATGGCCTGACCCTT
GAGCAGGCGGGGCTCGCCAAGAAGCAGGGCGGCTTGCCGGACGACTACGTCAGCGAGGTGCTGCAACCGTTTCGCGAGGC
GCTGGTACAGCAGGTGTCGCGCTCCCTGCAGTTCTTTTTCGCTTCCGGCCAGTACAACGCCGTTGACCATATTCTGCTGG
CGGGCGGCACGGCGTCGGTGCCCGGGCTGGACCGGCTGATCGAGGAACGCCTGAATACCCCGACCCAGGTCGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCGAGCGATGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J2PGW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

49.577

100

0.497