Detailed information NTG0485976

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JAB4_RS05625
• Genome accession: NZ_CP062062
• Coordinates: 1240050..1241189 (-)
• Length: 379 a.a.
• NCBI ID: WP_175444773.1
• Uniprot ID: A0A853SXI4
• Organism: Janthinobacterium sp. HH102
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1235050..1246189

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JAB4_RS05610 (JAB4_011060)	glcF	1236042..1237277 (+)	1236	WP_070222607.1	glycolate oxidase subunit GlcF	-
JAB4_RS05615 (JAB4_011070)	-	1237270..1239378 (-)	2109	WP_070222604.1	EAL domain-containing protein	-
JAB4_RS05620 (JAB4_011080)	-	1239563..1240048 (-)	486	WP_070222603.1	glutathione peroxidase	-
JAB4_RS05625 (JAB4_011090)	pilU	1240050..1241189 (-)	1140	WP_175444773.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
JAB4_RS05630 (JAB4_011100)	pilT	1241193..1242236 (-)	1044	WP_070222601.1	type IV pilus twitching motility protein PilT	Machinery gene
JAB4_RS05635 (JAB4_011110)	-	1242307..1243008 (+)	702	WP_070222600.1	YggS family pyridoxal phosphate-dependent enzyme	-
JAB4_RS05640 (JAB4_011120)	proC	1243025..1243855 (+)	831	WP_070222597.1	pyrroline-5-carboxylate reductase	-
JAB4_RS05645 (JAB4_011130)	-	1243868..1244584 (-)	717	WP_070222596.1	anti-sigma factor	-
JAB4_RS05650 (JAB4_011140)	-	1244581..1245180 (-)	600	WP_227742100.1	sigma-70 family RNA polymerase sigma factor	-
JAB4_RS05655 (JAB4_011150)	-	1245238..1245795 (-)	558	WP_070222591.1	DUF3455 domain-containing protein	-

Sequence

Protein

• Length: 379 a.a.
• Molecular weight: 40948.10 Da
• Isoelectric Point: 7.0447

>NTDB_id=485976 JAB4_RS05625 WP_175444773.1 1240050..1241189(-) (pilU) [Janthinobacterium sp. HH102]
MQTTHEHYGAMHALLAQMRARGGSDLFITAGFPPAIKLDGKLTPLAGGALAAQQAAGYVRAVMNERQAAEFASSREANFA
ISPDGLGRFRVSAFVQMGQAGMVLRLINTAIPTLDGLGLPAILQDIVMSKRGLVIMVGATGCGKSTTLAAMVGHRNAHSH
GHIITIEDPVEFIHPHGNCIVTQREVGVDTDDWATALKNTLRQAPDVIQIGEIRDRDTMDHAIAFAETGHLCLATLHANN
ANQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLIPNKEGGGRKAALEILLNSPLMSDLIFKGQVHEIKELMKKSREH
GMQTFDQALFDLHEAGAISYEDALRNADSVNDLRLAIKLKGATAPEPAPQTGATKLGLL

Nucleotide

• Length: 1140 bp

>NTDB_id=485976 JAB4_RS05625 WP_175444773.1 1240050..1241189(-) (pilU) [Janthinobacterium sp. HH102]
ATGCAGACGACGCACGAACACTATGGCGCCATGCACGCGCTGCTGGCGCAGATGCGCGCACGGGGCGGCTCCGACCTGTT
CATCACGGCCGGCTTTCCGCCCGCCATCAAGCTCGACGGCAAGCTGACGCCGCTGGCGGGCGGCGCGCTGGCCGCGCAGC
AGGCGGCCGGCTACGTGCGCGCCGTCATGAACGAACGCCAGGCGGCCGAGTTCGCATCGAGCCGCGAAGCCAATTTCGCC
ATCAGCCCGGACGGCCTCGGGCGTTTCCGCGTGTCCGCCTTCGTGCAGATGGGCCAGGCAGGCATGGTCTTGCGATTGAT
CAATACCGCCATCCCCACCCTGGATGGCCTGGGCCTGCCCGCCATCCTGCAAGACATCGTGATGAGCAAGCGGGGCCTCG
TCATCATGGTGGGCGCCACGGGCTGCGGCAAGTCGACCACCCTGGCCGCCATGGTGGGCCACCGCAACGCACACAGCCAT
GGCCACATCATCACCATCGAAGACCCCGTGGAATTCATCCATCCGCACGGCAACTGCATCGTCACCCAGCGCGAGGTGGG
CGTCGATACGGACGACTGGGCCACGGCCCTGAAAAACACCTTGCGGCAAGCGCCCGACGTGATCCAGATCGGCGAAATAC
GCGACCGCGACACGATGGACCACGCCATCGCGTTTGCCGAGACGGGCCACCTGTGCCTGGCGACTCTGCACGCAAACAAC
GCCAACCAGGCGCTGGACCGCATCATCAATTTCTTCCCGGAAGAGCGTCGCCAGCAACTGCTGATGGATCTGTCGCTGAA
TTTGAAGGGCATGATTTCGCAGCGTTTGATCCCCAACAAGGAAGGCGGCGGGCGCAAGGCGGCGCTGGAAATCCTGCTCA
ATTCGCCGCTGATGAGCGACCTGATCTTCAAGGGTCAGGTGCACGAGATCAAGGAATTGATGAAAAAATCGCGCGAGCAT
GGCATGCAGACGTTCGACCAGGCCCTGTTCGACCTGCACGAAGCGGGCGCCATCAGCTACGAAGACGCGCTGCGCAATGC
GGACTCCGTCAACGACCTGCGCCTGGCGATCAAACTCAAGGGCGCCACGGCGCCGGAACCCGCCCCGCAAACGGGCGCCA
CCAAACTGGGACTCCTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (88-278)

3D structure

• AlphaFold DB: A0A853SXI4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	63.456	93.14	0.591
pilU	Acinetobacter baylyi ADP1	59.366	91.557	0.544
pilU	Vibrio cholerae strain A1552	56.851	90.501	0.515
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.425	86.28	0.375