Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KBP48_RS10235 Genome accession   NZ_CP072932
Coordinates   2281759..2283492 (+) Length   577 a.a.
NCBI ID   WP_140189468.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain BB08-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2276759..2288492
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBP48_RS10215 (KBP48_10215) pilC 2277087..2278289 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  KBP48_RS10220 (KBP48_10220) pilA 2278730..2279176 (+) 447 WP_071869805.1 pilin Machinery gene
  KBP48_RS10225 (KBP48_10225) - 2279421..2279648 (-) 228 WP_012338102.1 hypothetical protein -
  KBP48_RS10230 (KBP48_10230) pilA/pilAI 2280481..2280924 (+) 444 WP_140189469.1 pilin Machinery gene
  KBP48_RS10235 (KBP48_10235) pilB 2281759..2283492 (+) 1734 WP_140189468.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KBP48_RS10240 (KBP48_10240) - 2283586..2284998 (-) 1413 WP_020851962.1 sigma-54 dependent transcriptional regulator -
  KBP48_RS10245 (KBP48_10245) pilS 2285185..2286795 (-) 1611 WP_004084601.1 ATP-binding protein Regulator
  KBP48_RS10250 (KBP48_10250) sucC 2287050..2288213 (+) 1164 WP_004084603.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63044.23 Da        Isoelectric Point: 6.2669

>NTDB_id=485841 KBP48_RS10235 WP_140189468.1 2281759..2283492(+) (pilB) [Xylella fastidiosa subsp. multiplex strain BB08-1]
MKTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSAAKLAAANALEFGMPLLDVSAFDHN
HNAMNLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALE
EEDNGIEGLDIGGGDDDGASGDTGIDAKSDDTPVVKFINKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPVQLPK
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGITGLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=485841 KBP48_RS10235 WP_140189468.1 2281759..2283492(+) (pilB) [Xylella fastidiosa subsp. multiplex strain BB08-1]
ATGAAAACACCTCATTCAGCCAATCTTGTCGGCATCACCGGTATTGCCCGTCGCCTGGTCCAGGATGGCAGCATTGAGGA
AGCCACTGCACGCACTGCTCAAAATCAGGCAATGGCCGCAAAAGTGCCTTTACCACAGTGGTTGATCGATAAAAAACTAG
TGAGTGCAGCAAAATTGGCCGCGGCCAATGCACTTGAGTTCGGGATGCCGCTGCTGGATGTGTCCGCATTCGACCACAAT
CACAATGCAATGAACTTGGTCGACGAAGAATTACTGCAAAAGCATCGGGTGTTGCCATTATTTAAACGCGGTAATCGACT
GTTCGTCGGGATCAGCAATCCAACCCAAACACAGGCCCTGGACGACATCAAATTCCACACCAATCTGGTCGTGGAACCGA
TTCTGGTCGACGAAGACCAGATACGACGCACACTTGAACAATGGCAATCTAGGAGCGGCGCGCTCAGCTCCGCATTGGAG
GAAGAGGATAACGGCATTGAAGGGCTGGATATCGGCGGCGGAGACGACGACGGGGCCAGTGGCGATACAGGGATTGATGC
CAAGAGCGATGACACACCTGTCGTCAAATTCATCAACAAAGTGCTGATTGATGCCATCCGACGCGGCGCATCAGACATCC
ACTTTGAACCATACGAAGAAGATTACCGCGTCCGTTTCCGGATTGATGGTCTGCTGAAGGCTGTGGCAAAAGCACCGAAC
AAGTTGAATCAGCGTATCGCCGCACGCTTGAAAGTCATGTCGCAACTGGATATCGCGGAAAAACGCGTCCCTCAAGACGG
ACGTATCAAGCTTAATCTGTCGAAAACCAAGCAAGTCGATTTCCGCGTCAGCACCTTGCCAACGCTGTTCGGCGAGAAAG
TGGTACTGCGTATCTTGGATGGCAGCGCGGCAAAGCTGGGTATCGACAAACTCGGTTACGAAGCTGACCAACAAACGCTT
TTTATGGAAGCAATCCACAAACCCTATGGAATGGTACTGGTCACTGGTCCGACCGGTTCGGGCAAGACCGTGTCACTGTA
CACTGCACTAGGCATCCTCAATGACGAAACACGCAATATCTCCACAGCCGAAGATCCTGTAGAAATCCGGCTGCCGGGTG
TCAATCAGGTACAACAAAACAACAAGCGCGGTATGACGTTTGCCGCAGCATTACGTTCGTTTCTGCGCCAAGACCCGGAT
GTGATCATGGTCGGCGAAATCCGGGATCTGGAAACCGCTGAAATCGCCATCAAAGCCGCACAGACCGGCCACATGGTGCT
CTCCACACTACATACCAACGATGCACCGCAAACCATCGCACGCCTCATGAACATGGGGATTGCACCATACAACATCACTT
CATCCGTGACCTTAGTGATTGCACAGCGATTGGCACGGAGATTGTGCAACAAGTGCAAGCGCCCGGTTCAATTGCCGAAA
AACGCATTACTGGCCGAAGGATTCACTCAAGAACAAATTGATGCGGGGCTGGAATTATACGAGCCTGTCGGTTGCGACGA
ATGCACCGAAGGATACAAAGGCCGCACAGGTATTTATCAAGTGATGCCAATTACCGAAGGGATTTCCTCGATCATTTTAG
CCGGCGGTAATGCCTTACAAATCGCCGAAGAGGCACAGCGCATCGGCATCAACGACCTACGCCAATCGGCGCTACTCAAA
GTCGCTCAGGGCATCACCGGGCTGGCTGAAATTAATCGAGTCACCAAAGATTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.319

98.267

0.563
  pilB Acinetobacter baumannii D1279779

56.79

98.267

0.558
  pilB Legionella pneumophila strain ERS1305867

51.408

98.44

0.506
  pilF Neisseria gonorrhoeae MS11

50.615

98.614

0.499
  pilB Vibrio cholerae strain A1552

50

94.281

0.471
  pilB Vibrio campbellii strain DS40M4

46.914

98.267

0.461
  pilB Vibrio parahaemolyticus RIMD 2210633

46.749

98.614

0.461
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.806

92.894

0.36