Detailed information NTG0485298

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BAMOH1_RS12090
• Genome accession: NZ_CP061853
• Coordinates: 2530046..2530660 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus amyloliquefaciens strain MOH1-5b
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2525046..2535660

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAMOH1_RS12060 (BAMOH1_12060)	rpsT	2525350..2525616 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BAMOH1_RS12065 (BAMOH1_12065)	holA	2525633..2526674 (-)	1042	Protein_2351	DNA polymerase III subunit delta	-
BAMOH1_RS12070 (BAMOH1_12070)	-	2526714..2526864 (-)	151	Protein_2352	hypothetical protein	-
BAMOH1_RS12075 (BAMOH1_12075)	-	2526905..2527039 (+)	135	WP_003152870.1	YqzM family protein	-
BAMOH1_RS12080 (BAMOH1_12080)	comEC	2527058..2529409 (-)	2352	WP_174747051.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BAMOH1_RS12085 (BAMOH1_12085)	-	2529410..2529979 (-)	570	WP_003152868.1	ComE operon protein 2	-
BAMOH1_RS12090 (BAMOH1_12090)	comEA	2530046..2530660 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
BAMOH1_RS12095 (BAMOH1_12095)	comER	2530719..2531540 (+)	822	WP_014418420.1	late competence protein ComER	-
BAMOH1_RS12100 (BAMOH1_12100)	-	2531609..2532346 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
BAMOH1_RS12105 (BAMOH1_12105)	rsfS	2532343..2532699 (-)	357	WP_007408260.1	ribosome silencing factor	-
BAMOH1_RS12110 (BAMOH1_12110)	yqeK	2532717..2533277 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BAMOH1_RS12115 (BAMOH1_12115)	-	2533267..2533836 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
BAMOH1_RS12120 (BAMOH1_12120)	yhbY	2533847..2534137 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BAMOH1_RS12125 (BAMOH1_12125)	aroE	2534131..2534973 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=485298 BAMOH1_RS12090 WP_014418419.1 2530046..2530660(-) (comEA) [Bacillus amyloliquefaciens strain MOH1-5b]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=485298 BAMOH1_RS12090 WP_014418419.1 2530046..2530660(-) (comEA) [Bacillus amyloliquefaciens strain MOH1-5b]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363