Detailed information NTG0484012

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IC769_RS13985
• Genome accession: NZ_CP061672
• Coordinates: 2979419..2980537 (+)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain AS23
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2974419..2985537

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC769_RS13970 (IC769_13970)	-	2976586..2977428 (-)	843	WP_174744079.1	undecaprenyl-diphosphate phosphatase	-
IC769_RS13975 (IC769_13975)	-	2977535..2978227 (-)	693	WP_191108628.1	YggS family pyridoxal phosphate-dependent enzyme	-
IC769_RS13980 (IC769_13980)	pilT	2978354..2979391 (+)	1038	WP_019456823.1	type IV pilus twitching motility protein PilT	Machinery gene
IC769_RS13985 (IC769_13985)	pilU	2979419..2980537 (+)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IC769_RS13990 (IC769_13990)	fur	2980607..2981044 (-)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
IC769_RS13995 (IC769_13995)	-	2981156..2981554 (+)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
IC769_RS14000 (IC769_14000)	-	2981610..2981933 (-)	324	WP_191108629.1	RnfH family protein	-
IC769_RS14005 (IC769_14005)	-	2981933..2983000 (-)	1068	WP_191108630.1	hypothetical protein	-
IC769_RS14010 (IC769_14010)	-	2983153..2983608 (-)	456	WP_004703295.1	bacteriohemerythrin	-
IC769_RS14015 (IC769_14015)	-	2983859..2984707 (-)	849	WP_019456827.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=484012 IC769_RS13985 WP_004703285.1 2979419..2980537(+) (pilU) [Acinetobacter seifertii strain AS23]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=484012 IC769_RS13985 WP_004703285.1 2979419..2980537(+) (pilU) [Acinetobacter seifertii strain AS23]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAGAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
TATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGTGAAGTAATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTTGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAACGCGATATGCCAGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCCTCTGTGCTTAAAGATTTATCAATGACCAAACGCGGCATTATTATTTTTGTAGGCGCAACAG
GTACTGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATCATTACTATTGAA
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTCGGTATTGATACTGACTCGTTTGA
AATTGCTTTGAAAAATACTTTACGACAAGCACCCGATGTCATTTTAATTGGTGAGATCCGTTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTCAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAG
GCTTTATTTGACTTATATAAAACAGGTCAAATTACCTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTGACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

3D structure

• AlphaFold DB: N8S8R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401