Detailed information NTG0483990

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC771_RS18195
• Genome accession: NZ_CP061666
• Coordinates: 3788472..3789530 (-)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS25
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3783472..3794530

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC771_RS18170 (IC771_18165)	aroK	3783784..3784353 (-)	570	WP_004705195.1	shikimate kinase AroK	-
IC771_RS18175 (IC771_18170)	pilQ	3784365..3786491 (-)	2127	WP_151792203.1	type IV pilus secretin PilQ family protein	Machinery gene
IC771_RS18180 (IC771_18175)	pilP	3786553..3787080 (-)	528	WP_077168924.1	pilus assembly protein PilP	Machinery gene
IC771_RS18185 (IC771_18180)	pilO	3787091..3787831 (-)	741	WP_077168923.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC771_RS18190 (IC771_18185)	pilN	3787828..3788472 (-)	645	WP_004705190.1	PilN domain-containing protein	Machinery gene
IC771_RS18195 (IC771_18190)	pilM	3788472..3789530 (-)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC771_RS18200 (IC771_18195)	ponA	3789692..3792247 (+)	2556	WP_134262504.1	penicillin-binding protein PBP1a	-
IC771_RS18205 (IC771_18200)	-	3792265..3793089 (-)	825	WP_143221083.1	putative RNA methyltransferase	-
IC771_RS18210 (IC771_18205)	-	3793092..3793424 (-)	333	WP_077168921.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=483990 IC771_RS18195 WP_004705187.1 3788472..3789530(-) (pilM) [Acinetobacter seifertii strain AS25]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483990 IC771_RS18195 WP_004705187.1 3788472..3789530(-) (pilM) [Acinetobacter seifertii strain AS25]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGACTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCGGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCTTCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426