Detailed information NTG0483749

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC778_RS17925
• Genome accession: NZ_CP061634
• Coordinates: 3752351..3753409 (-)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS41
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3747351..3758409

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC778_RS17900 (IC778_17895)	aroK	3747663..3748232 (-)	570	WP_004705195.1	shikimate kinase AroK	-
IC778_RS17905 (IC778_17900)	pilQ	3748244..3750370 (-)	2127	WP_151792203.1	type IV pilus secretin PilQ family protein	Machinery gene
IC778_RS17910 (IC778_17905)	pilP	3750432..3750959 (-)	528	WP_077168924.1	pilus assembly protein PilP	Machinery gene
IC778_RS17915 (IC778_17910)	pilO	3750970..3751710 (-)	741	WP_077168923.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC778_RS17920 (IC778_17915)	pilN	3751707..3752351 (-)	645	WP_004705190.1	PilN domain-containing protein	Machinery gene
IC778_RS17925 (IC778_17920)	pilM	3752351..3753409 (-)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC778_RS17930 (IC778_17925)	ponA	3753571..3756126 (+)	2556	WP_134262504.1	penicillin-binding protein PBP1a	-
IC778_RS17935 (IC778_17930)	-	3756144..3756968 (-)	825	WP_143221083.1	putative RNA methyltransferase	-
IC778_RS17940 (IC778_17935)	-	3756971..3757303 (-)	333	WP_077168921.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=483749 IC778_RS17925 WP_004705187.1 3752351..3753409(-) (pilM) [Acinetobacter seifertii strain AS41]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483749 IC778_RS17925 WP_004705187.1 3752351..3753409(-) (pilM) [Acinetobacter seifertii strain AS41]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGACTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCGGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCTTCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426