Detailed information NTG0483546

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC783_RS01615
• Genome accession: NZ_CP061613
• Coordinates: 344557..345615 (+)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS51
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 339557..350615

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC783_RS01600 (IC783_01600)	-	340663..340995 (+)	333	WP_077168921.1	hypothetical protein	-
IC783_RS01605 (IC783_01605)	-	340998..341822 (+)	825	WP_143221083.1	putative RNA methyltransferase	-
IC783_RS01610 (IC783_01610)	ponA	341840..344395 (-)	2556	WP_134262504.1	penicillin-binding protein PBP1a	-
IC783_RS01615 (IC783_01615)	pilM	344557..345615 (+)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC783_RS01620 (IC783_01620)	pilN	345615..346259 (+)	645	WP_004705190.1	PilN domain-containing protein	Machinery gene
IC783_RS01625 (IC783_01625)	pilO	346256..346996 (+)	741	WP_077168923.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC783_RS01630 (IC783_01630)	pilP	347007..347534 (+)	528	WP_077168924.1	pilus assembly protein PilP	Machinery gene
IC783_RS01635 (IC783_01635)	pilQ	347596..349722 (+)	2127	WP_151792203.1	type IV pilus secretin PilQ family protein	Machinery gene
IC783_RS01640 (IC783_01640)	aroK	349734..350303 (+)	570	WP_004705195.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=483546 IC783_RS01615 WP_004705187.1 344557..345615(+) (pilM) [Acinetobacter seifertii strain AS51]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483546 IC783_RS01615 WP_004705187.1 344557..345615(+) (pilM) [Acinetobacter seifertii strain AS51]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGACTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCGGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCTTCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426