Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IC785_RS16080 Genome accession   NZ_CP061603
Coordinates   3297331..3298449 (+) Length   372 a.a.
NCBI ID   WP_004703285.1    Uniprot ID   N8S8R6
Organism   Acinetobacter seifertii strain AS53     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3292331..3303449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC785_RS16055 (IC785_16055) tnpB 3292421..3292756 (+) 336 WP_000618090.1 IS66 family insertion sequence element accessory protein TnpB -
  IC785_RS16060 (IC785_16060) - 3292831..3294477 (+) 1647 WP_001094416.1 IS66-like element ISAba17 family transposase -
  IC785_RS16065 (IC785_16065) - 3294474..3295340 (-) 867 WP_190980281.1 undecaprenyl-diphosphate phosphatase -
  IC785_RS16070 (IC785_16070) - 3295447..3296139 (-) 693 WP_077170049.1 YggS family pyridoxal phosphate-dependent enzyme -
  IC785_RS16075 (IC785_16075) pilT 3296266..3297303 (+) 1038 WP_019456823.1 type IV pilus twitching motility protein PilT Machinery gene
  IC785_RS16080 (IC785_16080) pilU 3297331..3298449 (+) 1119 WP_004703285.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IC785_RS16085 (IC785_16085) fur 3298519..3298956 (-) 438 WP_004703287.1 ferric iron uptake transcriptional regulator -
  IC785_RS16090 (IC785_16090) - 3299068..3299466 (+) 399 WP_004703289.1 outer membrane protein assembly factor BamE -
  IC785_RS16095 (IC785_16095) - 3299522..3299845 (-) 324 WP_019456825.1 RnfH family protein -
  IC785_RS16100 (IC785_16100) - 3299845..3300912 (-) 1068 WP_143221137.1 hypothetical protein -
  IC785_RS16105 (IC785_16105) - 3301065..3301520 (-) 456 WP_004703295.1 bacteriohemerythrin -
  IC785_RS16110 (IC785_16110) - 3301771..3302619 (-) 849 WP_077170048.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41966.15 Da        Isoelectric Point: 6.6944

>NTDB_id=483525 IC785_RS16080 WP_004703285.1 3297331..3298449(+) (pilU) [Acinetobacter seifertii strain AS53]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=483525 IC785_RS16080 WP_004703285.1 3297331..3298449(+) (pilU) [Acinetobacter seifertii strain AS53]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTAATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTCGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAGCGTGATATGCCGGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCTTCTGTGCTTAAAGATTTATCAATGACCAAACGTGGCATTATTATTTTTGTAGGCGCAACAG
GTACAGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATTATTACTATTGAA
GACCCTATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTAGGTATTGATACTGACTCGTTCGA
AATTGCTTTAAAAAATACGTTACGACAAGCGCCCGATGTCATCTTAATTGGTGAGATCCGCTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTTAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTAACTTTTGATCGACAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(36-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N8S8R6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.132

100

0.763
  pilU Pseudomonas stutzeri DSM 10701

68.478

98.925

0.677
  pilU Vibrio cholerae strain A1552

55.271

94.355

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.972

95.43

0.401