Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IC800_RS16695 Genome accession   NZ_CP061543
Coordinates   3547947..3549659 (+) Length   570 a.a.
NCBI ID   WP_191110239.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS80     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3542947..3554659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC800_RS16660 (IC800_16660) nusA 3543836..3545320 (-) 1485 WP_004699068.1 transcription termination factor NusA -
  IC800_RS16665 (IC800_16665) rimP 3545358..3545882 (-) 525 WP_002157009.1 ribosome maturation factor RimP -
  IC800_RS16685 (IC800_16685) secG 3546520..3546849 (-) 330 WP_002156913.1 preprotein translocase subunit SecG -
  IC800_RS16690 (IC800_16690) tpiA 3546862..3547656 (-) 795 WP_191107091.1 triose-phosphate isomerase -
  IC800_RS16695 (IC800_16695) pilB 3547947..3549659 (+) 1713 WP_191110239.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC800_RS16700 (IC800_16700) pilC 3549687..3550913 (+) 1227 WP_191107092.1 type II secretion system F family protein Machinery gene
  IC800_RS16705 (IC800_16705) - 3550913..3551774 (+) 862 Protein_3296 A24 family peptidase -
  IC800_RS16710 (IC800_16710) coaE 3551776..3552372 (+) 597 WP_039251211.1 dephospho-CoA kinase -
  IC800_RS16715 (IC800_16715) - 3552375..3553283 (-) 909 WP_191107094.1 DMT family transporter -
  IC800_RS16720 (IC800_16720) rlmB 3553315..3554067 (-) 753 WP_191000795.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IC800_RS16725 (IC800_16725) - 3554170..3554496 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63497.79 Da        Isoelectric Point: 5.0862

>NTDB_id=483052 IC800_RS16695 WP_191110239.1 3547947..3549659(+) (pilB) [Acinetobacter seifertii strain AS80]
MSALHTSPKFTGFFRRLVEEEHVSATTMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKVLNQHFAEESSFDFNDEEF
DLDVSLDSPATQEDEDETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKTPTDVPKQSLLEMGF
TEQDLAHPDFLVFQPVGCPECRDGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGLTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=483052 IC800_RS16695 WP_191110239.1 3547947..3549659(+) (pilB) [Acinetobacter seifertii strain AS80]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAGAACATGTGTCGGCAACGAC
CATGCAAACTGCATTAGATGCAGCAAAAAGAGCTAAGCGGGATACAGTTGCCTATTTAATTGAAGAGGTACATCTCTCCC
CTTCTTTATTGGCTGAAACAATTTCCGCTGAGTTTGCTGAACCATATTTTGATCTTGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCACTTATTCAAAGAGGCCAAATTTTATATGT
TGCGACTAGCAATCCAAGCAATATTGAAGCGATTGATGCGATTCGTTTTAATAGCAAACTGATGGTTGAGCCTGTGATTG
TCGAGCACCATAAACTCGAAAAAGTTTTAAACCAACATTTCGCAGAAGAAAGCAGTTTTGATTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATAGCCCTGCCACTCAAGAAGATGAAGACGAAACACCTCAAGGTGATGAAGCTCCCATTGT
TAAATATATTAATAAGTTGCTCATTGATGCAATTCGTATGGGGGCTTCAGATTTACATTTTGAACCGTATGAAAAGTCAT
ATCGGGTTCGATATCGTGTCGACGGCGTGTTACGTCAAATTGCCAATCCTCCTTTACAATTGGCAAACCGTTTGGCTTCA
CGTTTAAAAGTAATGTCTCAAATGGATATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCGACGTTGTTTGGTGAAAAGCTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGGATTGACGCATTAGGATACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAAGGCATGCTTTTAATTACTGGACCAACAGGTTCCGGTAAAACAGTATCTTTATATACAGGTTTAAACATTTTAAATAC
TGAGAGCTCTAATATTTCTACTGCCGAAGACCCTGTAGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTGAACCCAA
AAGTAGGTCTCACTTTTGCTGCTGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATCGCGATTAAAGCTGCACAAACGGGTCACATGGTGATGTCAACACTGCATACCAACAGTGC
ACCAGAAACACTGACTCGCTTACGCAATATGGGGGTTCCTTCTTTCAATATTGCAACATCAGTCAACTTGGTTATTGCTC
AGCGGTTAGCACGCCGCCTTTGCTCTCAATGTAAAACACCTACAGATGTTCCAAAACAAAGTCTATTAGAAATGGGCTTT
ACCGAGCAGGACTTAGCACATCCGGACTTTCTGGTTTTCCAACCTGTAGGTTGCCCTGAATGTCGTGATGGCTATAAAGG
CCGTGTCGGTATTTATGAAGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTAATGCAAGGGTTGACCTCT
CTACAAGAAGTCAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

96.491

100

0.965
  pilB Acinetobacter baylyi ADP1

79.298

100

0.793
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio cholerae strain A1552

47.703

99.298

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

51.136

92.632

0.474
  pilB Vibrio campbellii strain DS40M4

50.669

91.754

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384