Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ICG51_RS05260 Genome accession   NZ_CP061497
Coordinates   1105971..1107695 (+) Length   574 a.a.
NCBI ID   WP_190281958.1    Uniprot ID   A0A7H1RKI0
Organism   Thermomonas sp. XSG     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1093072..1115324 1105971..1107695 within 0


Gene organization within MGE regions


Location: 1093072..1115324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICG51_RS05200 (ICG51_001041) - 1093072..1094955 (+) 1884 WP_190281947.1 hypothetical protein -
  ICG51_RS05205 (ICG51_001042) - 1094969..1096912 (+) 1944 WP_190281948.1 acyltransferase family protein -
  ICG51_RS05210 (ICG51_001043) - 1096981..1097769 (+) 789 WP_190281949.1 hypothetical protein -
  ICG51_RS05215 (ICG51_001044) - 1097766..1097897 (+) 132 Protein_1017 GDP-mannose 4,6-dehydratase -
  ICG51_RS05220 (ICG51_001045) - 1097943..1099187 (+) 1245 WP_190281950.1 oligosaccharide flippase family protein -
  ICG51_RS05225 (ICG51_001046) - 1099209..1100072 (+) 864 WP_190281951.1 FkbM family methyltransferase -
  ICG51_RS05230 (ICG51_001047) - 1100077..1101198 (+) 1122 WP_223809524.1 glycosyltransferase -
  ICG51_RS05235 (ICG51_001048) - 1101169..1102005 (-) 837 WP_190281953.1 glycosyltransferase -
  ICG51_RS05240 (ICG51_001049) - 1102002..1103030 (-) 1029 WP_190281954.1 glycosyltransferase family 4 protein -
  ICG51_RS05245 (ICG51_001050) - 1103020..1103751 (-) 732 WP_190281955.1 class I SAM-dependent methyltransferase -
  ICG51_RS05250 (ICG51_001051) - 1103748..1104869 (-) 1122 WP_190281956.1 glycosyltransferase family 4 protein -
  ICG51_RS05255 (ICG51_001052) - 1104931..1105794 (-) 864 WP_190281957.1 glycosyltransferase family 2 protein -
  ICG51_RS05260 (ICG51_001053) pilB 1105971..1107695 (+) 1725 WP_190281958.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ICG51_RS05265 (ICG51_001054) pilC 1107707..1108975 (+) 1269 WP_190281959.1 type II secretion system F family protein Machinery gene
  ICG51_RS05270 (ICG51_001055) - 1108994..1109857 (+) 864 WP_190281960.1 A24 family peptidase -
  ICG51_RS05275 (ICG51_001056) coaE 1109918..1110535 (+) 618 WP_190281961.1 dephospho-CoA kinase -
  ICG51_RS05280 (ICG51_001057) - 1110618..1110995 (-) 378 Protein_1030 NUDIX domain-containing protein -
  ICG51_RS05285 (ICG51_001058) secA 1111005..1113722 (-) 2718 WP_190281963.1 preprotein translocase subunit SecA -
  ICG51_RS05290 (ICG51_001059) - 1113866..1114807 (-) 942 WP_190281964.1 M23 family metallopeptidase -
  ICG51_RS05295 (ICG51_001060) - 1114812..1115324 (+) 513 WP_190282369.1 DUF721 domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62544.00 Da        Isoelectric Point: 6.5155

>NTDB_id=482592 ICG51_RS05260 WP_190281958.1 1105971..1107695(+) (pilB) [Thermomonas sp. XSG]
MSTAPTANLMGITGIARRLVLDGALDEAVARKAMDGATAEKMPMASYLADRRLVTPGALAAANSIEFGMPLVDALAIDPA
QSAITLVKEELLRKHQVLPLFKRGNRLFVGTADPTNNRALDDIKFQTNLTVEPILVDADRIKRCLDQWLEAADAMADDLA
DADGLDGLEVGGGDDDLSSDTGVDAKGEDTPVVKFVNKALVDAIRRGASDIHFEPYETDYRVRFRIDGILKTFKKVPVKL
HARIAARLKVMAQLDIAEKRIPQDGRIKLNISKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKMGIEKLGYEPDQQKLFL
DSIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTVEDPVEIRLPGVNQVQQNAKRGMTFAAALRSFLRQDPDVI
MVGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVNLVIAQRLVRRLHDCKRAVHLPEHAL
AAEGFTADEIHAGITVYEAVGCEECTGGYKGRAGIYQVMPMTEEIQQIVLAGGNVQQLTEASLRSGVRDLRRSALDKVKQ
GITSLIEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=482592 ICG51_RS05260 WP_190281958.1 1105971..1107695(+) (pilB) [Thermomonas sp. XSG]
ATGAGCACCGCCCCCACTGCCAATCTGATGGGCATCACCGGGATCGCCCGGCGCCTGGTGCTGGACGGCGCCCTGGACGA
GGCCGTCGCGCGCAAGGCGATGGACGGCGCGACCGCCGAGAAGATGCCGATGGCGTCCTACCTGGCCGATCGCCGGCTGG
TGACGCCCGGGGCACTGGCAGCCGCCAACTCCATCGAGTTCGGCATGCCGCTGGTGGATGCGCTGGCCATCGACCCGGCC
CAGTCCGCGATCACGCTGGTCAAGGAAGAGTTGCTGCGCAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGCT
GTTCGTGGGCACGGCCGACCCCACCAACAACCGCGCGCTGGATGACATCAAGTTCCAGACCAACCTGACGGTGGAACCGA
TCCTGGTGGACGCCGACCGGATCAAGCGTTGCCTGGACCAGTGGCTGGAGGCCGCCGACGCGATGGCCGACGACCTGGCC
GACGCCGACGGTCTGGACGGCCTGGAGGTCGGCGGCGGCGACGACGACCTGAGCAGCGATACCGGCGTGGACGCCAAGGG
CGAGGACACCCCGGTCGTCAAGTTCGTCAACAAGGCGCTGGTGGACGCCATCCGCCGCGGCGCCTCGGACATCCACTTCG
AGCCGTACGAGACGGACTACCGGGTGCGCTTCCGCATCGACGGCATCCTGAAGACGTTCAAGAAGGTGCCGGTGAAGCTG
CACGCGCGCATCGCCGCGCGCCTGAAGGTGATGGCCCAGCTGGATATCGCCGAAAAGCGCATCCCGCAGGACGGCCGCAT
CAAGCTCAACATCAGTAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCCACCCTGTTCGGCGAGAAGATCGTGC
TGCGTATCCTGGACGGCAGCGCCGCCAAGATGGGCATCGAGAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTGTTCCTG
GACAGTATCCACAAGCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGCTCCGGCAAGACGGTGAGCCTGTACACCGC
GCTGGGCATCCTCAACGACGAGACCCGCAACATCAGCACGGTCGAGGACCCGGTGGAAATCCGCCTGCCGGGCGTCAACC
AGGTGCAGCAGAACGCCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGACCCGGACGTGATC
ATGGTGGGCGAAATCCGCGACCTGGAAACCGCGGAGATCGCGGTGAAGGCGGCGCAGACCGGCCACATGGTGCTGTCCAC
CCTGCACACCAACGACGCGCCGCAGACCATCGCGCGCTTGATGAACATGGGCATCGCGCCCTACAACATCACCAGCTCGG
TGAACCTGGTCATCGCCCAGCGCCTGGTGCGCCGCCTGCACGACTGCAAGCGCGCGGTGCACCTGCCCGAGCACGCCCTG
GCGGCTGAAGGCTTCACTGCCGACGAGATCCACGCCGGCATCACGGTCTATGAGGCGGTGGGTTGCGAGGAGTGCACCGG
CGGCTACAAGGGCCGCGCCGGCATCTACCAGGTGATGCCGATGACCGAGGAAATCCAGCAGATCGTGCTGGCTGGTGGTA
ATGTCCAGCAGCTGACCGAGGCTTCCCTGCGCAGCGGCGTCCGCGATCTGCGGCGCTCAGCGCTGGACAAGGTGAAACAG
GGGATCACCAGCCTCATCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H1RKI0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.993

98.955

0.524
  pilB Acinetobacter baylyi ADP1

52.92

98.432

0.521
  pilB Legionella pneumophila strain ERS1305867

50.088

98.78

0.495
  pilF Neisseria gonorrhoeae MS11

48.708

94.425

0.46
  pilB Vibrio cholerae strain A1552

51.267

89.373

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

45.975

97.387

0.448
  pilB Vibrio campbellii strain DS40M4

49.315

89.024

0.439
  pilF Thermus thermophilus HB27

40.23

90.941

0.366