Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NMAA_RS10055 Genome accession   NC_017512
Coordinates   1912983..1914659 (-) Length   558 a.a.
NCBI ID   WP_002245855.1    Uniprot ID   -
Organism   Neisseria meningitidis WUE 2594     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1907983..1919659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMAA_RS10035 (NMAA_1656) pilG 1909896..1911128 (+) 1233 WP_002216405.1 type 4 pilus assembly protein PilG Machinery gene
  NMAA_RS10040 (NMAA_1657) pilD 1911202..1912062 (+) 861 WP_002220276.1 prepilin peptidase Machinery gene
  NMAA_RS10045 (NMAA_1658) coaE 1912064..1912696 (+) 633 WP_002245856.1 dephospho-CoA kinase -
  NMAA_RS10050 (NMAA_1659) - 1912689..1912898 (+) 210 WP_002212333.1 DNA gyrase inhibitor YacG -
  NMAA_RS10055 (NMAA_1660) pilF 1912983..1914659 (-) 1677 WP_002245855.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NMAA_RS10065 (NMAA_1661) - 1915101..1915547 (-) 447 WP_002233503.1 DUF441 domain-containing protein -
  NMAA_RS10070 (NMAA_1662) ispB 1915552..1916526 (-) 975 WP_002216396.1 octaprenyl diphosphate synthase -
  NMAA_RS10075 (NMAA_1663) rplU 1916750..1917058 (+) 309 WP_002216394.1 50S ribosomal protein L21 -
  NMAA_RS10080 (NMAA_1664) rpmA 1917083..1917355 (+) 273 WP_002212328.1 50S ribosomal protein L27 -
  NMAA_RS15860 - 1917408..1917530 (+) 123 Protein_1895 IS5/IS1182 family transposase -
  NMAA_RS10085 (NMAA_1665) ubiM 1917694..1918878 (+) 1185 WP_002239495.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  NMAA_RS10090 (NMAA_1666) rpmG 1919149..1919304 (-) 156 WP_002212306.1 50S ribosomal protein L33 -
  NMAA_RS10095 (NMAA_1667) rpmB 1919336..1919569 (-) 234 WP_002216391.1 50S ribosomal protein L28 -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61995.07 Da        Isoelectric Point: 5.3037

>NTDB_id=47879 NMAA_RS10055 WP_002245855.1 1912983..1914659(-) (pilF) [Neisseria meningitidis WUE 2594]
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQSLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=47879 NMAA_RS10055 WP_002245855.1 1912983..1914659(-) (pilF) [Neisseria meningitidis WUE 2594]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGAGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAGACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATCCATTTCGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCCTACGGGATGGTGCTGGTAACCGGTCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGAGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGCTTCACCGATGAGGACCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

99.283

100

0.993
  pilB Acinetobacter baumannii D1279779

50.088

100

0.509
  pilB Acinetobacter baylyi ADP1

50.178

100

0.505
  pilB Legionella pneumophila strain ERS1305867

47.35

100

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.457
  pilB Vibrio cholerae strain A1552

45.196

100

0.455
  pilB Vibrio campbellii strain DS40M4

43.662

100

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.771

93.728

0.373

Multiple sequence alignment