Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HP15_RS12020 Genome accession   NC_017506
Coordinates   2543581..2545296 (+) Length   571 a.a.
NCBI ID   WP_014577712.1    Uniprot ID   E4PH70
Organism   Marinobacter adhaerens HP15     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2538581..2550296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HP15_RS11995 (HP15_2405) cysQ 2538597..2539373 (-) 777 WP_014577708.1 3'(2'),5'-bisphosphate nucleotidase CysQ -
  HP15_RS12000 (HP15_2406) - 2539464..2540825 (+) 1362 WP_014577709.1 MBL fold metallo-hydrolase RNA specificity domain-containing protein -
  HP15_RS22910 - 2540848..2541246 (-) 399 WP_041645354.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HP15_RS12010 (HP15_2407) - 2541332..2542606 (-) 1275 WP_041645356.1 O-antigen ligase -
  HP15_RS23015 (HP15_2408) - 2542858..2543268 (-) 411 WP_014577711.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HP15_RS12020 (HP15_2409) pilB 2543581..2545296 (+) 1716 WP_014577712.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HP15_RS12025 (HP15_2410) pilC 2545299..2546519 (+) 1221 WP_014577713.1 type II secretion system F family protein Machinery gene
  HP15_RS12030 (HP15_2411) pilD 2546520..2547395 (+) 876 WP_014577714.1 A24 family peptidase Machinery gene
  HP15_RS12035 (HP15_2412) coaE 2547399..2547998 (+) 600 WP_014577715.1 dephospho-CoA kinase -
  HP15_RS12040 (HP15_2413) - 2547991..2549085 (+) 1095 WP_014577716.1 hypothetical protein -
  HP15_RS12045 (HP15_2414) tsaA 2549085..2549828 (+) 744 WP_014577717.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62636.47 Da        Isoelectric Point: 4.8170

>NTDB_id=47834 HP15_RS12020 WP_014577712.1 2543581..2545296(+) (pilB) [Marinobacter adhaerens HP15]
MATDSSKVTLTGLARRFVEDGLLDEATAKDAFIQASQNRIPLITYLVQNNLADSSGLAFSAAMEFGVSVLDLSAFATEMI
PEKVVDEKLIRKHNALPIYKRGNRLFIAVSDPTNIQALDEFKFNTGLSTDAVLVDDAKLRDSIEKYLESQDTSMGDLEDA
DLEGVETEGGEQEDDNAVSASDVDDAPIVKYVNKMLLDAIRGGASDVHFEPYEKVYRVRYRTDGILKEVSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKQIYLEALE
QPQGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPAEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSVIIAQRLGRRLCNSCKQAADIPKDVLLAE
GFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAKQAQAEGFRNLRQAALLKVIQGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=47834 HP15_RS12020 WP_014577712.1 2543581..2545296(+) (pilB) [Marinobacter adhaerens HP15]
ATGGCAACTGACAGCAGCAAAGTAACTCTCACCGGCCTGGCACGACGTTTCGTTGAGGACGGGCTTCTAGATGAAGCGAC
TGCCAAAGACGCATTCATTCAAGCCTCACAAAACCGAATACCGTTGATCACCTATCTGGTTCAGAACAACCTGGCGGATA
GCAGCGGGCTGGCATTCTCGGCTGCCATGGAATTCGGTGTTTCTGTATTGGATCTCTCGGCGTTTGCGACGGAAATGATT
CCGGAGAAGGTGGTCGACGAAAAGCTGATCCGCAAGCACAACGCTCTGCCTATTTACAAGCGCGGAAACCGGCTGTTTAT
TGCTGTTTCTGACCCGACCAATATCCAGGCGCTTGATGAGTTCAAATTCAACACCGGCCTGAGTACCGATGCGGTATTGG
TAGACGACGCAAAACTCCGTGACTCCATTGAAAAATACCTGGAATCTCAGGATACCTCCATGGGGGATCTGGAGGATGCA
GACCTTGAGGGTGTAGAGACAGAGGGCGGCGAACAGGAAGACGACAACGCTGTTTCTGCCAGTGATGTCGACGATGCACC
TATCGTGAAGTATGTGAACAAAATGTTGCTGGATGCGATTCGCGGCGGCGCATCGGATGTGCATTTCGAACCTTATGAAA
AGGTTTATCGGGTTCGCTACCGCACGGACGGTATTTTGAAAGAAGTGTCGCGGCCTTCCATCAAATTGGCGCCCAAAATT
TCTGCCCGGGTAAAAATCATGGCGCAGCTGGACATTTCCGAACGGCGTGTACCTCAGGATGGACGGATTAAAATGAAACT
GTCCAAAACCAAGGCCATCGACTTCCGGGTAAACACACTACCCACTCTATGGGGCGAAAAGATCGTATTGCGGATACTGG
ATCCGAGCCAGGCCAAGCTGGGCATTGATGTTCTTGGTTACGAGGAAGACCAGAAGCAGATTTATCTTGAGGCCCTCGAA
CAACCCCAAGGTATGATTCTGGTAACCGGGCCAACTGGCTCAGGTAAAACGGTTTCGCTCTACACCGGCCTGAACATCCT
AAACACTGCCGAAAGGAATATCTCAACTGCCGAGGACCCGGCCGAGATTAACCTTGAAGGGATCAATCAGGTAAACGTGA
ACACCCGAGTCGGGCTCGGCTTCGCCGAAGCATTGCGAGCCTTCCTCCGCCAGGACCCCGACGTAATCATGGTGGGTGAG
ATTCGGGACCTGGAGACCGCCAACATCGCGATCAAGGCCGCACAAACCGGCCACCTTGTTCTTTCCACACTGCACACCAA
CAGTGCCGCCGAAACGCTCACGCGGATGATGAACATGGGTGTTCCCTCATTCAACATCGCCACTTCGGTAAGTGTGATTA
TCGCGCAGCGATTGGGCAGGCGGTTGTGTAACAGCTGCAAGCAAGCCGCAGACATCCCCAAAGACGTGCTTTTGGCGGAG
GGGTTCACACAGGAACAAATTGATACAGGCTTCACGTTGTACCGCCCAAAGGGCTGTGATAAATGCAATGGGGGATACAA
AGGCCGCGTTGGTATTTACGAGGTGGTCAAGATTACCGACGAGCTGGCAAACATGATTATGGAAGAGGCCAGTTCCATCA
AAATTGCAAAACAGGCTCAGGCAGAGGGCTTCCGTAACTTGCGGCAGGCAGCGCTTCTGAAGGTGATTCAGGGTGTGACC
AGTCTTGAGGAAGCCAATCGCGTGACGAAGGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4PH70

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.168

100

0.573
  pilB Acinetobacter baylyi ADP1

55.42

100

0.555
  pilB Legionella pneumophila strain ERS1305867

53.217

100

0.536
  pilB Vibrio parahaemolyticus RIMD 2210633

51.619

97.373

0.503
  pilB Vibrio cholerae strain A1552

54.423

91.068

0.496
  pilB Vibrio campbellii strain DS40M4

50.177

98.774

0.496
  pilF Neisseria gonorrhoeae MS11

48.142

98.949

0.476
  pilB/pilB1 Synechocystis sp. PCC 6803

39.279

97.198

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.304

92.119

0.371

Multiple sequence alignment