Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H9L16_RS08060 Genome accession   NZ_CP060719
Coordinates   1756863..1758587 (-) Length   574 a.a.
NCBI ID   WP_187551214.1    Uniprot ID   A0A7G9SLI5
Organism   Thermomonas carbonis strain KCTC 42013     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1751863..1763587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9L16_RS08040 (H9L16_08040) - 1752994..1753962 (+) 969 WP_187551207.1 Nudix family hydrolase -
  H9L16_RS08045 (H9L16_08045) coaE 1753959..1754564 (-) 606 WP_187551209.1 dephospho-CoA kinase -
  H9L16_RS08050 (H9L16_08050) - 1754689..1755552 (-) 864 WP_187551211.1 A24 family peptidase -
  H9L16_RS08055 (H9L16_08055) pilC 1755584..1756852 (-) 1269 WP_187551213.1 type II secretion system F family protein Machinery gene
  H9L16_RS08060 (H9L16_08060) pilB 1756863..1758587 (-) 1725 WP_187551214.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H9L16_RS08065 (H9L16_08065) - 1758621..1761686 (-) 3066 WP_187551217.1 fused MFS/spermidine synthase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62626.12 Da        Isoelectric Point: 6.2557

>NTDB_id=478018 H9L16_RS08060 WP_187551214.1 1756863..1758587(-) (pilB) [Thermomonas carbonis strain KCTC 42013]
MSAVTSANLMGITGIARRLVLDGALEEAAARKAMDSATAEKQPLAAYLAERRLVTPAAIAAANSIEFGMPLVDALALDPS
QSAIALVKEELLRKHQVLPLFKRGGRLFVGTSDPTDHQALDELKFHTNLAVEPILVDAERIKRCLDQWLEVAEAMADEMA
DSEGLEGLEVSGGDDDLSNDTGVDAKGEDTPVVKFINKALVDAIRKGASDIHFEPYETDYRVRFRIDGILKLAKRAPVKL
HQRIAARLKVMSQLDIAEKRIPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPIQQKLFM
DAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTVEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVSLVIAQRLLRRLHDCKRAVHLPEHAL
LAEGFTADEVHAGITVYEAVGCEDCTEGYKGRAGVYQVMPMNDEIQQIVLAGGNVQQITEASLRSGVRDLRGSALDKVKQ
GITSLIEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=478018 H9L16_RS08060 WP_187551214.1 1756863..1758587(-) (pilB) [Thermomonas carbonis strain KCTC 42013]
ATGAGCGCAGTCACGTCCGCCAATTTGATGGGAATTACCGGCATTGCCCGGCGTCTGGTGCTGGATGGCGCGCTGGAAGA
GGCCGCTGCGCGCAAGGCGATGGATTCCGCCACCGCAGAGAAGCAGCCGCTGGCGGCTTATCTTGCCGAGCGCAGGCTGG
TCACGCCCGCGGCGATCGCGGCTGCCAATTCCATCGAATTCGGCATGCCGCTGGTGGATGCGCTGGCGCTCGACCCGTCG
CAGTCGGCGATTGCGCTGGTCAAGGAAGAATTGCTGCGCAAGCACCAGGTCCTGCCGCTGTTCAAACGCGGTGGACGCCT
GTTCGTGGGCACCAGCGACCCGACCGACCACCAGGCGCTGGACGAACTGAAGTTCCACACCAACCTGGCGGTGGAGCCGA
TCCTGGTCGATGCCGAGCGCATCAAGCGCTGCCTGGACCAGTGGCTGGAAGTCGCAGAGGCGATGGCCGACGAGATGGCC
GATTCCGAAGGCCTGGAAGGCCTGGAAGTCAGCGGCGGCGATGACGACCTCAGCAACGACACCGGCGTGGACGCCAAGGG
CGAAGACACGCCCGTGGTCAAGTTCATCAACAAGGCGCTGGTCGATGCGATCCGCAAGGGCGCCAGCGACATCCACTTCG
AGCCCTACGAAACCGACTATCGGGTCAGGTTCCGCATCGACGGCATCCTCAAACTGGCCAAGCGGGCACCGGTCAAGCTG
CACCAGCGCATTGCCGCGCGCCTGAAGGTCATGTCGCAGCTGGATATCGCCGAGAAGCGCATTCCGCAGGACGGTCGCAT
CAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCGACCCTGTTCGGCGAGAAGATCGTGC
TGCGCATCCTGGACGGCAGCGCCGCCAAGCTGGGTATCGACAAGCTGGGTTACGAGCCGATCCAGCAGAAACTGTTCATG
GATGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCGGGCAAGACCGTCAGTCTGTATACCGC
GCTGGGCATCCTCAACGACGAGACCCGCAACATCAGCACGGTCGAGGATCCGGTGGAAATTCGCCTGCCCGGGGTCAACC
AGGTGCAGCAGAACGTCAAGCGCGGGATGACCTTCGCCGCGGCACTGCGCAGTTTCCTGCGCCAGGATCCCGACATCATC
ATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGAGATCGCGGTCAAAGCCGCACAGACCGGCCACATGGTGTTGTCCAC
CCTGCACACCAACGACGCGCCGCAGACCATCGCACGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCAGTTCGG
TCAGCCTGGTGATTGCCCAGCGCCTGCTGCGTCGCCTGCACGACTGCAAGCGCGCGGTGCACCTGCCCGAGCATGCCCTG
TTGGCCGAGGGCTTCACCGCGGACGAAGTGCATGCTGGCATCACCGTCTACGAGGCGGTGGGCTGCGAGGACTGCACCGA
GGGCTACAAGGGCCGTGCCGGCGTGTACCAGGTGATGCCCATGAACGACGAAATCCAGCAGATCGTGCTGGCCGGTGGTA
ATGTCCAGCAAATCACCGAGGCGTCGCTCCGCAGCGGAGTCCGCGACCTGCGTGGTTCAGCGTTGGACAAGGTGAAACAG
GGGATCACCAGTCTCATCGAGATCAACCGAGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-149)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G9SLI5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.167

100

0.544
  pilB Acinetobacter baumannii D1279779

54.69

98.432

0.538
  pilB Legionella pneumophila strain ERS1305867

51.056

98.955

0.505
  pilF Neisseria gonorrhoeae MS11

48.587

98.606

0.479
  pilB Vibrio cholerae strain A1552

52.047

89.373

0.465
  pilB Vibrio campbellii strain DS40M4

50.61

85.714

0.434
  pilB Vibrio parahaemolyticus RIMD 2210633

50.61

85.714

0.434