Detailed information NTG0477948

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: H9L17_RS10780
• Genome accession: NZ_CP060711
• Coordinates: 2341162..2342886 (-)
• Length: 574 a.a.
• NCBI ID: WP_187569454.1
• Uniprot ID: A0A7G9QQR1
• Organism: Thermomonas brevis strain KACC 16975
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2343854..2355891	2341162..2342886	flank	968

Gene organization within MGE regions

Location: 2341162..2355891

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H9L17_RS10780 (H9L17_10775)	pilB	2341162..2342886 (-)	1725	WP_187569454.1	type IV-A pilus assembly ATPase PilB	Machinery gene
H9L17_RS10785 (H9L17_10780)	-	2343004..2343861 (+)	858	WP_187569455.1	glycosyltransferase family 2 protein	-
H9L17_RS10790 (H9L17_10785)	-	2343875..2345011 (+)	1137	WP_246455076.1	glycosyltransferase family 4 protein	-
H9L17_RS10795 (H9L17_10790)	-	2345390..2346304 (-)	915	WP_187569457.1	glycosyltransferase	-
H9L17_RS10800 (H9L17_10795)	-	2346460..2347380 (-)	921	WP_187569458.1	hypothetical protein	-
H9L17_RS10805 (H9L17_10800)	-	2347499..2348218 (-)	720	WP_187569459.1	class I SAM-dependent methyltransferase	-
H9L17_RS10810 (H9L17_10805)	-	2348215..2349039 (-)	825	WP_187569460.1	methyltransferase, TIGR04325 family	-
H9L17_RS10815 (H9L17_10810)	-	2349296..2350645 (-)	1350	WP_187569461.1	oligosaccharide flippase family protein	-
H9L17_RS10820 (H9L17_10815)	-	2350747..2350947 (-)	201	WP_187569462.1	integrase core domain-containing protein	-
H9L17_RS10825 (H9L17_10820)	-	2351271..2351636 (-)	366	WP_187569463.1	SGNH hydrolase domain-containing protein	-
H9L17_RS10830 (H9L17_10825)	-	2351820..2352416 (-)	597	WP_187569464.1	acyltransferase	-
H9L17_RS10835 (H9L17_10830)	-	2352507..2353064 (-)	558	WP_187569465.1	acyltransferase family protein	-
H9L17_RS10840 (H9L17_10835)	-	2352970..2355015 (-)	2046	WP_187569466.1	hypothetical protein	-
H9L17_RS15925	-	2355328..2355420 (-)	93	Protein_2135	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
H9L17_RS16065	-	2355802..2355891 (-)	90	Protein_2136	prepilin-type N-terminal cleavage/methylation domain-containing protein	-

Sequence

Protein

• Length: 574 a.a.
• Molecular weight: 62682.95 Da
• Isoelectric Point: 6.3870

>NTDB_id=477948 H9L17_RS10780 WP_187569454.1 2341162..2342886(-) (pilB) [Thermomonas brevis strain KACC 16975]
MNTAASPNLMGITGIARRLVMDGALDEAAARRAMDNATAEKTPLASYLADHRLASPAALAAANSVEFGMPLLDALAIDPA
QSAIALVKEELLRKHNVLPLFKRGNRLFVGTSDPTSNRAMEELKFHTNLAVEPILVDAERIKRSLDQWLEAAEALADELD
DAEGLEGLEVGGGDDDLSNDTGVDAKGEDTPVVKFVNKALVDAIRRGASDIHFEPYETDYRVRFRIDGILKTTKKVPVKL
HSRIAARLKVMSQLDIAEKRIPQDGRIKLNISKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKMGIEKLGYEPEQQKLFL
DAIHKPYGMVLVTGPTGSGKTVSLYTGLGILNDETRNISTVEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDVI
MVGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVNLVIAQRLVRRLHDCKRAVHLPEHAL
LAEGFTADEIHQGITVYEAVGCEDCTGGYKGRAGIYQVMPMTDEIQQIVLAGGNVQQLTEASLRSGVRDLRRSALDKVKQ
GITSLIEINRVTKD

Nucleotide

• Length: 1725 bp

>NTDB_id=477948 H9L17_RS10780 WP_187569454.1 2341162..2342886(-) (pilB) [Thermomonas brevis strain KACC 16975]
ATGAATACCGCCGCTTCCCCCAACCTGATGGGCATCACCGGCATCGCCAGACGTCTGGTGATGGACGGCGCCCTGGACGA
AGCTGCTGCCCGCAGGGCGATGGACAACGCCACGGCGGAGAAGACCCCGCTGGCCAGCTACCTGGCCGACCATCGGCTGG
CCTCGCCGGCAGCGCTGGCCGCAGCCAACTCGGTCGAGTTCGGCATGCCGCTGCTGGATGCGCTGGCCATCGATCCGGCC
CAGTCCGCCATCGCACTGGTCAAGGAAGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGCAATCGGCT
GTTCGTCGGCACGTCCGACCCCACCAGCAACCGGGCCATGGAAGAACTCAAGTTCCACACCAATCTGGCGGTGGAGCCGA
TTCTGGTGGATGCCGAACGCATCAAGCGCAGCCTGGATCAGTGGCTGGAGGCAGCGGAAGCGCTGGCCGACGAGCTGGAC
GATGCAGAAGGCCTGGAAGGCCTGGAAGTCGGGGGCGGCGACGACGACTTGAGCAATGACACCGGCGTGGATGCCAAGGG
TGAAGACACCCCGGTGGTGAAGTTCGTGAACAAAGCGCTGGTGGATGCGATCCGCCGCGGCGCCTCGGACATCCACTTCG
AGCCCTACGAAACCGATTACCGGGTGCGTTTCCGCATCGACGGCATCCTGAAGACCACCAAGAAGGTGCCGGTGAAGCTG
CACTCGCGCATCGCCGCGCGGCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCATCCCGCAAGACGGCCGCAT
CAAGCTGAACATCAGCAAGACCAAGCAGATCGACTTCCGCGTCAGTACCCTGCCCACCCTGTTCGGCGAGAAGATCGTGC
TGCGCATCCTGGACGGCAGCGCCGCCAAGATGGGCATCGAAAAGTTGGGCTACGAGCCAGAGCAGCAGAAGCTGTTCCTG
GACGCCATCCACAAACCCTATGGCATGGTGCTGGTGACCGGCCCCACCGGCTCCGGCAAGACCGTCAGCCTGTACACCGG
ACTGGGCATCCTCAACGACGAGACCCGCAACATCAGCACGGTGGAGGACCCGGTGGAAATCCGCCTGCCGGGCGTCAATC
AGGTGCAGCAGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCCCTGCGCAGCTTCCTGCGCCAGGATCCGGACGTGATC
ATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCGGTCAAGGCGGCGCAGACCGGCCACATGGTGCTGTCCAC
CCTGCACACCAACGACGCCCCGCAGACCATCGCCCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGTTCGG
TCAACCTGGTGATCGCCCAGCGCCTGGTGCGCCGCCTGCACGACTGCAAGCGCGCCGTCCACTTGCCGGAGCACGCCCTG
CTGGCCGAGGGCTTCACCGCCGACGAGATTCACCAAGGCATCACCGTCTACGAGGCAGTGGGCTGCGAGGACTGCACCGG
CGGCTACAAGGGGCGCGCCGGCATCTACCAGGTGATGCCGATGACGGATGAAATCCAGCAAATCGTGCTGGCTGGTGGTA
ATGTCCAGCAGCTGACCGAGGCGTCCCTGCGCAGCGGCGTCCGCGACCTGCGGCGCTCCGCGTTGGACAAGGTGAAACAG
GGGATCACCAGCCTCATCGAGATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (198-465)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (48-153)

3D structure

• AlphaFold DB: A0A7G9QQR1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	54.401	98.955	0.538
pilB	Acinetobacter baumannii D1279779	53.451	98.432	0.526
pilB	Legionella pneumophila strain ERS1305867	51.408	98.955	0.509
pilF	Neisseria gonorrhoeae MS11	48.233	98.606	0.476
pilB	Vibrio cholerae strain A1552	52.047	89.373	0.465
pilB	Vibrio campbellii strain DS40M4	49.119	89.024	0.437
pilB	Vibrio parahaemolyticus RIMD 2210633	49.797	85.714	0.427
pilF	Thermus thermophilus HB27	39.655	90.941	0.361