Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   H7795_RS00695 Genome accession   NZ_CP060641
Coordinates   106854..107288 (+) Length   144 a.a.
NCBI ID   WP_023612536.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain TSPY210     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 101854..112288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7795_RS00665 (H7795_00660) - 103523..103888 (+) 366 WP_002986560.1 DUF1033 family protein -
  H7795_RS00670 (H7795_00665) comYA 103981..104919 (+) 939 WP_023613135.1 competence type IV pilus ATPase ComGA Machinery gene
  H7795_RS00675 (H7795_00670) comYB 104855..105889 (+) 1035 WP_258855794.1 competence type IV pilus assembly protein ComGB Machinery gene
  H7795_RS00680 (H7795_00675) comYC 105891..106217 (+) 327 WP_032465607.1 competence type IV pilus major pilin ComGC Machinery gene
  H7795_RS00685 (H7795_00680) comGD 106192..106620 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  H7795_RS00690 (H7795_00685) comGE 106577..106861 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  H7795_RS00695 (H7795_00690) comYF 106854..107288 (+) 435 WP_023612536.1 competence type IV pilus minor pilin ComGF Machinery gene
  H7795_RS00700 (H7795_00695) comGG 107272..107598 (+) 327 WP_002992739.1 competence type IV pilus minor pilin ComGG -
  H7795_RS00705 (H7795_00700) comYH 107696..108649 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  H7795_RS00710 (H7795_00705) - 108708..109904 (+) 1197 WP_023612537.1 acetate kinase -
  H7795_RS00715 (H7795_00710) - 110091..110399 (+) 309 WP_030126458.1 hypothetical protein -
  H7795_RS00720 (H7795_00715) proC 110482..111252 (-) 771 WP_002986527.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16688.29 Da        Isoelectric Point: 10.2526

>NTDB_id=477074 H7795_RS00695 WP_023612536.1 106854..107288(+) (comYF) [Streptococcus pyogenes strain TSPY210]
MSKQLSNIKAFTFLEALIALLVISGSLLVYQGLTRTLLKHSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=477074 H7795_RS00695 WP_023612536.1 106854..107288(+) (comYF) [Streptococcus pyogenes strain TSPY210]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCTTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCGAACCCTCCTTAAACATAGCCATTATCTAGCCCGTCATGATCAAGATAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

50.36

96.528

0.486
  comYF Streptococcus mutans UA159

49.64

96.528

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

43.284

93.056

0.403
  comGF/cglF Streptococcus pneumoniae Rx1

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae D39

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae R6

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae TIGR4

42.105

92.361

0.389
  comGF/cglF Streptococcus mitis SK321

41.353

92.361

0.382