Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   J1F34_RS12315 Genome accession   NZ_CP071441
Coordinates   2549778..2551016 (-) Length   412 a.a.
NCBI ID   WP_000815976.1    Uniprot ID   B7L4R8
Organism   Escherichia coli strain EcPF20     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2544778..2556016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1F34_RS12295 (J1F34_12295) dam 2545390..2546226 (-) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -
  J1F34_RS12300 (J1F34_12300) damX 2546333..2547619 (-) 1287 WP_000343193.1 cell division protein DamX -
  J1F34_RS12305 (J1F34_12305) aroB 2547711..2548799 (-) 1089 WP_000439846.1 3-dehydroquinate synthase -
  J1F34_RS12310 (J1F34_12310) aroK 2548856..2549377 (-) 522 WP_000818618.1 shikimate kinase AroK -
  J1F34_RS12315 (J1F34_12315) comE 2549778..2551016 (-) 1239 WP_000815976.1 DNA uptake porin HofQ Machinery gene
  J1F34_RS12320 (J1F34_12320) hofP 2550928..2551332 (-) 405 WP_001264141.1 DNA utilization protein HofP -
  J1F34_RS12325 (J1F34_12325) hofO 2551322..2551762 (-) 441 WP_001055760.1 DNA utilization protein HofO -
  J1F34_RS12330 (J1F34_12330) hofN 2551746..2552285 (-) 540 WP_001069315.1 DNA utilization protein HofN -
  J1F34_RS12335 (J1F34_12335) hofM 2552285..2553064 (-) 780 WP_001315880.1 DNA utilization protein HofM -
  J1F34_RS12340 (J1F34_12340) mrcA 2553184..2555736 (+) 2553 WP_001367029.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44717.22 Da        Isoelectric Point: 6.1173

>NTDB_id=477005 J1F34_RS12315 WP_000815976.1 2549778..2551016(-) (comE) [Escherichia coli strain EcPF20]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNDNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=477005 J1F34_RS12315 WP_000815976.1 2549778..2551016(-) (comE) [Escherichia coli strain EcPF20]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTGGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGGCTG
ATAACGCGCCAGGAGGGCAACATTCTCTCAGTGCATTCTATTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTTCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGTGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGTATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L4R8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362