Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   J0G23_RS12480 Genome accession   NZ_CP071263
Coordinates   2517292..2518530 (-) Length   412 a.a.
NCBI ID   WP_071594129.1    Uniprot ID   -
Organism   Escherichia coli strain MEI003     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2512292..2523530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0G23_RS12460 (J0G23_12385) dam 2512904..2513740 (-) 837 WP_000742139.1 adenine-specific DNA-methyltransferase -
  J0G23_RS12465 (J0G23_12390) damX 2513847..2515133 (-) 1287 WP_024229585.1 cell division protein DamX -
  J0G23_RS12470 (J0G23_12395) aroB 2515225..2516313 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  J0G23_RS12475 (J0G23_12400) aroK 2516370..2516891 (-) 522 WP_000818618.1 shikimate kinase AroK -
  J0G23_RS12480 (J0G23_12405) comE 2517292..2518530 (-) 1239 WP_071594129.1 DNA uptake porin HofQ Machinery gene
  J0G23_RS12485 (J0G23_12410) hofP 2518442..2518846 (-) 405 WP_001560037.1 DNA utilization protein HofP -
  J0G23_RS12490 (J0G23_12415) hofO 2518836..2519276 (-) 441 WP_000367364.1 DNA utilization protein HofO -
  J0G23_RS12495 (J0G23_12420) hofN 2519260..2519799 (-) 540 WP_001560039.1 DNA utilization protein HofN -
  J0G23_RS12500 (J0G23_12425) hofM 2519799..2520578 (-) 780 WP_001445781.1 DNA utilization protein HofM -
  J0G23_RS12505 (J0G23_12430) mrcA 2520698..2523250 (+) 2553 WP_001301905.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44708.21 Da        Isoelectric Point: 5.9488

>NTDB_id=476444 J0G23_RS12480 WP_071594129.1 2517292..2518530(-) (comE) [Escherichia coli strain MEI003]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNDNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=476444 J0G23_RS12480 WP_071594129.1 2517292..2518530(-) (comE) [Escherichia coli strain MEI003]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAAGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGTCAGGAGGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATGACAATATCGCCCGTCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCCGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGTTGGCTGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCTCATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATCCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTTACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGTTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362