Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   LCGL_RS12035 Genome accession   NC_017490
Coordinates   421756..423789 (+) Length   677 a.a.
NCBI ID   WP_016170993.1    Uniprot ID   -
Organism   Lactococcus garvieae Lg2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416756..428789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCGL_RS12020 (LCGL_0400) - 417956..419512 (+) 1557 WP_004259192.1 ABC-F family ATP-binding cassette domain-containing protein -
  LCGL_RS12025 (LCGL_0401) - 419678..420853 (+) 1176 WP_004259189.1 SLC13 family permease -
  LCGL_RS12030 (LCGL_0402) - 420911..421561 (+) 651 WP_014024335.1 helix-hairpin-helix domain-containing protein -
  LCGL_RS12035 (LCGL_0404) comEC 421756..423789 (+) 2034 WP_016170993.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LCGL_RS12040 (LCGL_0405) - 423944..424720 (+) 777 WP_014024338.1 alpha/beta hydrolase -
  LCGL_RS12045 (LCGL_0406) - 424916..425131 (+) 216 WP_003135074.1 F0F1 ATP synthase subunit C -
  LCGL_RS12050 (LCGL_0407) atpB 425186..425899 (+) 714 WP_004259176.1 F0F1 ATP synthase subunit A -
  LCGL_RS12055 (LCGL_0408) atpF 425913..426419 (+) 507 WP_004259173.1 F0F1 ATP synthase subunit B -
  LCGL_RS12060 (LCGL_0409) - 426434..426961 (+) 528 WP_014024339.1 F0F1 ATP synthase subunit delta -
  LCGL_RS12065 (LCGL_0410) atpA 427027..428535 (+) 1509 WP_004259170.1 F0F1 ATP synthase subunit alpha -

Sequence

Protein


Download         Length: 677 a.a.        Molecular weight: 77899.96 Da        Isoelectric Point: 9.6151

>NTDB_id=47618 LCGL_RS12035 WP_016170993.1 421756..423789(+) (comEC) [Lactococcus garvieae Lg2]
MIKKETEVQERKQPEKISKITPYMDTLQVNGSRLSFRGEVNGNDFQAFYTLTSEKEKEYFKKLAVKGTFYLEGTLEIPEE
QRNFSGFDYRKYLRTQGVYRLLKIEKIYGFEEKQDFDLRLVRRKAILWTQEHFPSPMSSYMTGLLFGWLDKDFEEMGELY
TSLGIIHLFALSGMQVNFFIELLRNILLRLGLSQKLVFFIQIPFSIFYAFLTGLSLSILRALLQKNIPLKNSSDRFSVTF
LLLMVFSPYFLLTTGGQLTMLYSFLITFFSGKFKELKGLKKSLLDSTVLAVGVLPLLIFHFHSFQPLSIILTLLFSLIFD
FLLLPGLLFVFLLSLFGPALTLFNPFFIVLESIVRWVGDLFDQPIVFGKPNLIFLLLLFMLSGLALDFYKKRNWNLSIIM
VLFLLFFFVKNPKHPSITMVDVGQGDSILLQDSWNSQNILIDTGGRLKIRADEQWKEGIYKSNAENTLLPYLRARGVSKI
DVLIVTHPDEDHIGDLESVAQKVPIKNVYVSASALEKKSFAKKLSRLNSQIHIVKEGDKLPIFNSYLWFLTTGVEGKSDN
DNSLVTFGEFYGKKFLFTGDLEEEGEMQLQEEYPHLEVDVLKVGHHGSKTSSKETFLEAINPKLALISAGKNNRYKHPNQ
ETLQQFAARGIKIYRTDQQGAIRLLYKKGSWHIQTVK

Nucleotide


Download         Length: 2034 bp        

>NTDB_id=47618 LCGL_RS12035 WP_016170993.1 421756..423789(+) (comEC) [Lactococcus garvieae Lg2]
ATGATAAAAAAAGAAACGGAAGTACAAGAAAGAAAGCAACCAGAAAAAATTTCGAAAATTACCCCTTATATGGATACACT
TCAAGTAAATGGAAGCAGGCTCAGTTTCAGAGGAGAAGTGAACGGCAACGACTTCCAAGCTTTTTACACCTTGACCTCGG
AAAAAGAAAAAGAATACTTCAAAAAACTTGCGGTGAAGGGGACTTTTTACCTTGAAGGCACTTTGGAGATTCCAGAAGAG
CAAAGAAATTTTTCAGGTTTTGATTACAGGAAGTATTTAAGGACTCAAGGGGTCTATCGTCTTCTTAAAATAGAAAAAAT
TTATGGATTTGAAGAAAAACAGGACTTTGATTTACGGCTGGTACGCCGAAAAGCCATTCTTTGGACTCAGGAACATTTCC
CTTCTCCTATGTCGTCCTATATGACAGGGCTTCTTTTTGGGTGGTTGGATAAAGACTTTGAAGAAATGGGGGAGCTTTAC
ACTAGCCTTGGTATTATCCATTTATTTGCGCTGTCAGGCATGCAGGTGAATTTTTTTATTGAACTTCTCCGAAATATTTT
ACTGCGGCTTGGCTTATCACAGAAGTTAGTCTTTTTTATCCAAATTCCCTTTTCGATATTTTATGCATTTCTTACAGGCT
TATCTCTTTCAATCTTACGTGCGCTTCTTCAAAAAAATATCCCCCTTAAAAATTCAAGCGATAGATTTTCTGTCACTTTT
CTTCTTTTAATGGTGTTCAGTCCTTATTTCCTGCTGACAACAGGAGGACAACTCACGATGCTTTATTCTTTCTTAATCAC
ATTTTTTTCAGGAAAGTTCAAAGAACTAAAAGGATTAAAAAAATCGTTACTTGATTCTACTGTCCTAGCTGTTGGGGTTC
TTCCGCTTTTGATTTTTCACTTCCATAGTTTTCAGCCATTATCGATTATCTTGACTTTATTATTTAGTCTGATTTTTGAT
TTTTTACTTTTGCCAGGTTTACTTTTTGTTTTTCTTCTTTCTTTATTCGGTCCTGCTCTCACGTTATTTAATCCTTTTTT
TATTGTTTTAGAATCAATAGTAAGATGGGTGGGCGATTTATTTGACCAGCCGATTGTCTTTGGCAAACCCAACCTTATTT
TTTTACTTTTACTTTTTATGCTGAGTGGACTTGCCCTTGATTTTTATAAAAAAAGAAACTGGAACTTGAGCATAATAATG
GTTCTTTTCTTGCTTTTTTTCTTTGTTAAGAATCCTAAACACCCAAGCATTACAATGGTAGATGTGGGACAAGGGGACAG
TATTCTTTTACAAGACAGTTGGAACAGCCAAAACATCCTCATCGATACGGGCGGGAGATTAAAAATAAGAGCTGATGAGC
AGTGGAAGGAGGGAATCTATAAGAGCAATGCCGAAAATACACTTCTGCCTTATCTGAGAGCGCGTGGCGTGAGTAAGATA
GATGTTCTGATTGTGACACATCCAGATGAGGATCATATTGGAGACCTGGAGAGTGTGGCTCAGAAAGTACCAATAAAAAA
TGTTTATGTGTCAGCTTCTGCATTGGAGAAGAAAAGCTTTGCTAAAAAACTCAGCCGTTTAAACAGTCAGATTCACATTG
TGAAGGAGGGGGACAAGCTCCCCATCTTTAACAGTTATCTCTGGTTTTTAACGACAGGTGTTGAAGGTAAAAGCGACAAT
GATAATTCTTTAGTGACATTTGGTGAATTTTATGGGAAGAAATTTCTTTTTACAGGCGACTTAGAAGAAGAGGGAGAGAT
GCAACTCCAGGAAGAATATCCCCATTTAGAGGTGGATGTCCTAAAAGTTGGACATCATGGCAGTAAAACTTCTTCAAAGG
AAACTTTTTTAGAAGCCATCAACCCTAAGCTCGCTCTTATTTCGGCTGGAAAGAATAATCGATATAAACATCCCAATCAA
GAAACGCTCCAACAATTCGCTGCACGAGGCATAAAAATTTATCGTACAGACCAGCAAGGAGCAATCCGTCTCCTTTATAA
AAAAGGCAGTTGGCACATTCAAACAGTAAAGTAG

Domains

Predicted by InterproScan.

Competence protein (Competence)

(144-386)

Metallo-beta-lactamase superfamily (Lactamase_B)

(422-630)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Lactococcus lactis subsp. cremoris KW2

54.491

98.671

0.538
  comEC/celB Streptococcus mitis SK321

47.111

99.705

0.47
  comEC/celB Streptococcus mitis NCTC 12261

45.562

99.852

0.455
  comEC/celB Streptococcus pneumoniae TIGR4

45.536

99.261

0.452
  comEC/celB Streptococcus pneumoniae Rx1

45.089

99.261

0.448
  comEC/celB Streptococcus pneumoniae D39

45.089

99.261

0.448
  comEC/celB Streptococcus pneumoniae R6

45.089

99.261

0.448

Multiple sequence alignment