Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   I653_RS13005 Genome accession   NC_020832
Coordinates   2609766..2610770 (-) Length   334 a.a.
NCBI ID   WP_003229718.1    Uniprot ID   O32055
Organism   Bacillus subtilis subsp. subtilis str. BAB-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2604766..2615770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I653_RS12975 (I653_13170) yrbG 2605740..2606396 (-) 657 WP_015483509.1 DUF421 domain-containing protein -
  I653_RS12980 (I653_13175) - 2606594..2606983 (+) 390 WP_015483510.1 TIGR04086 family membrane protein -
  I653_RS12985 (I653_13180) yajC 2607040..2607306 (-) 267 WP_004398708.1 preprotein translocase subunit YajC -
  I653_RS12990 (I653_13185) tgt 2607343..2608488 (-) 1146 WP_003229725.1 tRNA guanosine(34) transglycosylase Tgt -
  I653_RS12995 (I653_13190) queA 2608515..2609543 (-) 1029 WP_015483511.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  I653_RS13000 (I653_13195) yrzS 2609573..2609773 (-) 201 WP_015384252.1 DUF2905 domain-containing protein -
  I653_RS13005 (I653_13200) ruvB 2609766..2610770 (-) 1005 WP_003229718.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  I653_RS13010 (I653_13205) ruvA 2610781..2611386 (-) 606 WP_003229717.1 Holliday junction branch migration protein RuvA Machinery gene
  I653_RS13015 (I653_13210) bofC 2611525..2612037 (-) 513 WP_003229715.1 sporulation cell-cell signaling protein BofC -
  I653_RS13020 (I653_13215) csbX 2612085..2613392 (-) 1308 WP_015483512.1 MFS transporter -
  I653_RS13025 (I653_13220) iolG 2613463..2614488 (-) 1026 WP_015483513.1 inositol 2-dehydrogenase -
  I653_RS13030 (I653_13225) yrzF 2614726..2615373 (+) 648 WP_015483514.1 phosphotransferase -
  I653_RS21080 (I653_13230) - 2615419..2615541 (-) 123 WP_003229707.1 hypothetical protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37467.78 Da        Isoelectric Point: 5.1631

>NTDB_id=47506 I653_RS13005 WP_003229718.1 2609766..2610770(-) (ruvB) [Bacillus subtilis subsp. subtilis str. BAB-1]
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=47506 I653_RS13005 WP_003229718.1 2609766..2610770(-) (ruvB) [Bacillus subtilis subsp. subtilis str. BAB-1]
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCCATTGTAGCCAATGAAATGGGAGTGGAA
TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGGCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACG
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTGATGTCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT
ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

100

100

1
  ruvB Streptococcus pneumoniae TIGR4

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae R6

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae D39

60.429

97.605

0.59
  ruvB Synechocystis sp. PCC 6803

57.143

98.503

0.563
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518

Multiple sequence alignment