Detailed information NTG0474136

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: JRY11_RS04245
• Genome accession: NZ_CP070382
• Coordinates: 820260..820706 (+)
• Length: 148 a.a.
• NCBI ID: WP_029344525.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain KF140
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 815260..825706

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JRY11_RS04220 (JRY11_000852)	comGA	817336..818274 (+)	939	WP_031561106.1	competence type IV pilus ATPase ComGA	Machinery gene
JRY11_RS04225 (JRY11_000853)	comGB	818168..819241 (+)	1074	WP_074453836.1	competence type IV pilus assembly protein ComGB	Machinery gene
JRY11_RS04230 (JRY11_000854)	comGC	819369..819638 (+)	270	WP_023188581.1	competence type IV pilus major pilin ComGC	Machinery gene
JRY11_RS04235 (JRY11_000855)	comGD	819670..820029 (+)	360	WP_023188582.1	competence type IV pilus minor pilin ComGD	Machinery gene
JRY11_RS04240 (JRY11_000856)	comGE	820001..820297 (+)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
JRY11_RS04245 (JRY11_000857)	comGF	820260..820706 (+)	447	WP_029344525.1	competence type IV pilus minor pilin ComGF	Machinery gene
JRY11_RS04250 (JRY11_000858)	comGG	820745..821029 (+)	285	WP_025017139.1	competence type IV pilus minor pilin ComGG	Machinery gene
JRY11_RS04255 (JRY11_000859)	-	821127..821564 (+)	438	WP_003129992.1	zinc-dependent MarR family transcriptional regulator	-
JRY11_RS04260 (JRY11_000860)	-	821561..822403 (+)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
JRY11_RS04265 (JRY11_000861)	-	822580..823317 (+)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
JRY11_RS04270 (JRY11_000862)	-	823310..824119 (+)	810	WP_014570791.1	metal ABC transporter permease	-
JRY11_RS04275 (JRY11_000863)	-	824157..825023 (-)	867	WP_025017140.1	RluA family pseudouridine synthase	-
JRY11_RS04280 (JRY11_000864)	-	825228..825605 (+)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=474136 JRY11_RS04245 WP_029344525.1 820260..820706(+) (comGF) [Lactococcus lactis strain KF140]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=474136 JRY11_RS04245 WP_029344525.1 820260..820706(+) (comGF) [Lactococcus lactis strain KF140]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	93.919	0.716
comGF	Streptococcus mutans UA140	48.227	95.27	0.459
comGF	Streptococcus mutans UA159	47.518	95.27	0.453
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385