NTDB

Overview

Name	comE	Type	Machinery gene
Locus tag	I6K14_RS01145	Genome accession	NZ_CP070170
Coordinates	265154..266392 (+)	Length	412 a.a.
NCBI ID	WP_000816006.1	Uniprot ID	-
Organism	Escherichia coli strain FDAARGOS_1284
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 260154..271392

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6K14_RS01120 (I6K14_01120)	mrcA	260434..262986 (-)	2553	WP_001296475.1	peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA	-
I6K14_RS01125 (I6K14_01125)	hofM	263106..263885 (+)	780	WP_001350443.1	DNA utilization protein HofM	-
I6K14_RS01130 (I6K14_01130)	hofN	263885..264424 (+)	540	Protein_228	DNA utilization protein HofN	-
I6K14_RS01135 (I6K14_01135)	hofO	264408..264848 (+)	441	WP_001055744.1	DNA utilization protein HofO	-
I6K14_RS01140 (I6K14_01140)	hofP	264838..265242 (+)	405	WP_001264138.1	DNA utilization protein HofP	-
I6K14_RS01145 (I6K14_01145)	comE	265154..266392 (+)	1239	WP_000816006.1	DNA uptake porin HofQ	Machinery gene
I6K14_RS01150 (I6K14_01150)	aroK	266793..267314 (+)	522	WP_000818618.1	shikimate kinase AroK	-
I6K14_RS01155 (I6K14_01155)	aroB	267371..268459 (+)	1089	WP_000439850.1	3-dehydroquinate synthase	-
I6K14_RS01160 (I6K14_01160)	damX	268551..269837 (+)	1287	WP_087598215.1	cell division protein DamX	-
I6K14_RS01165 (I6K14_01165)	dam	269944..270780 (+)	837	WP_000742141.1	adenine-specific DNA-methyltransferase	-

Sequence

Protein

Download Length: 412 a.a. Molecular weight: 44797.26 Da Isoelectric Point: 5.9488

                                >NTDB_id=473442 I6K14_RS01145 WP_000816006.1 265154..266392(+) (comE) [Escherichia coli strain FDAARGOS_1284]

MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL

ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN

KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTTLGSDLSVATATTHIGFNIGRIN

GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL

HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL

VVFITPRLVSSE

Nucleotide

Download Length: 1239 bp

                                >NTDB_id=473442 I6K14_RS01145 WP_000816006.1 265154..266392(+) (comE) [Escherichia coli strain FDAARGOS_1284]

ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTTATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT

GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG

TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG

ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA

GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA

AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC

AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT

TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGTTGGCCGATGCGCAACAAGGTGGTGGCGTTG

GGCAAGTCACTACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC

GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC

CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT

CGGTAGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA

CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA

AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA

GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA

GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comE	Haemophilus influenzae 86-028NP	37.3	100	0.396
pilQ	Vibrio campbellii strain DS40M4	38.48	100	0.393
comE	Haemophilus influenzae Rd KW20	37.156	100	0.393
pilQ	Vibrio cholerae strain A1552	37.772	100	0.379
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	37.772	100	0.379
comE	Glaesserella parasuis strain SC1401	35.782	100	0.367
pilQ	Pseudomonas aeruginosa PAK	34.633	100	0.367

Detailed information NTG0473442