Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   I6K13_RS20400 Genome accession   NZ_CP070162
Coordinates   4166420..4167658 (-) Length   412 a.a.
NCBI ID   WP_000816006.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1283     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4161420..4172658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K13_RS20380 (I6K13_20375) dam 4162032..4162868 (-) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -
  I6K13_RS20385 (I6K13_20380) damX 4162975..4164261 (-) 1287 WP_021524563.1 cell division protein DamX -
  I6K13_RS20390 (I6K13_20385) aroB 4164353..4165441 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  I6K13_RS20395 (I6K13_20390) aroK 4165498..4166019 (-) 522 WP_000818618.1 shikimate kinase AroK -
  I6K13_RS20400 (I6K13_20395) comE 4166420..4167658 (-) 1239 WP_000816006.1 DNA uptake porin HofQ Machinery gene
  I6K13_RS20405 (I6K13_20400) hofP 4167570..4167974 (-) 405 WP_001264137.1 DNA utilization protein HofP -
  I6K13_RS20410 (I6K13_20405) hofO 4167964..4168404 (-) 441 WP_021524564.1 DNA utilization protein HofO -
  I6K13_RS20415 (I6K13_20410) hofN 4168388..4168927 (-) 540 WP_021524565.1 DNA utilization protein HofN -
  I6K13_RS20420 (I6K13_20415) hofM 4168927..4169706 (-) 780 WP_021524566.1 DNA utilization protein HofM -
  I6K13_RS20425 (I6K13_20420) mrcA 4169826..4172378 (+) 2553 Protein_4052 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44797.26 Da        Isoelectric Point: 5.9488

>NTDB_id=473408 I6K13_RS20400 WP_000816006.1 4166420..4167658(-) (comE) [Escherichia coli strain FDAARGOS_1283]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTTLGSDLSVATATTHIGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=473408 I6K13_RS20400 WP_000816006.1 4166420..4167658(-) (comE) [Escherichia coli strain FDAARGOS_1283]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG
ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCTGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGTTGGCCGATGCGCAACAAGGTGGTGGCGTTG
GGCAAGTCACTACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGTTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.3

100

0.396
  pilQ Vibrio campbellii strain DS40M4

38.48

100

0.393
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.782

100

0.367
  pilQ Pseudomonas aeruginosa PAK

34.633

100

0.367