Detailed information NTG0473175

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: HZS91_RS16640
• Genome accession: NZ_CP060002
• Coordinates: 3862625..3864361 (-)
• Length: 578 a.a.
• NCBI ID: WP_011052125.1
• Uniprot ID: A0AAI7ZH88
• Organism: Xanthomonas citri pv. citri strain SN3-3
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3843234..3861783	3862625..3864361	flank	842

Gene organization within MGE regions

Location: 3843234..3864361

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HZS91_RS16565 (HZS91_03500)	avrXacE2	3843234..3844304 (-)	1071	WP_011052114.1	type III secretion system effector avirulence protein AvrXacE2	-
HZS91_RS16570 (HZS91_03501)	-	3844389..3845666 (-)	1278	WP_011052115.1	lytic murein transglycosylase	-
HZS91_RS16575 (HZS91_03502)	-	3845799..3848789 (-)	2991	WP_219875336.1	Tn3 family transposase	-
HZS91_RS16580 (HZS91_03503)	-	3848798..3850072 (-)	1275	Protein_3249	site-specific integrase	-
HZS91_RS16585 (HZS91_03504)	-	3850264..3851319 (+)	1056	WP_219875338.1	DNA-binding protein	-
HZS91_RS16590 (HZS91_03505)	xopAI	3851452..3852342 (+)	891	WP_011052119.1	type III secretion system effector XopAI	-
HZS91_RS16595 (HZS91_03507)	-	3853673..3853957 (+)	285	WP_016849322.1	hypothetical protein	-
HZS91_RS16600 (HZS91_03508)	-	3854185..3855300 (+)	1116	WP_011052122.1	IS1595 family transposase	-
HZS91_RS16605 (HZS91_03509)	-	3855255..3855770 (-)	516	WP_011052123.1	hypothetical protein	-
HZS91_RS16610 (HZS91_03510)	sucD	3855857..3856732 (-)	876	WP_005921370.1	succinate--CoA ligase subunit alpha	-
HZS91_RS16615 (HZS91_03511)	sucC	3856757..3857926 (-)	1170	WP_005915812.1	ADP-forming succinate--CoA ligase subunit beta	-
HZS91_RS16620 (HZS91_03512)	-	3858158..3859771 (+)	1614	WP_003488599.1	HAMP domain-containing sensor histidine kinase	-
HZS91_RS16625 (HZS91_03513)	pilR	3860096..3861490 (+)	1395	WP_015463522.1	sigma-54 dependent transcriptional regulator	Regulator
HZS91_RS16630 (HZS91_03515)	-	3861713..3861880 (-)	168	WP_015463523.1	hypothetical protein	-
HZS91_RS16635 (HZS91_03516)	pilB	3862012..3862131 (-)	120	WP_005915819.1	hypothetical protein	Machinery gene
HZS91_RS16640 (HZS91_03518)	pilB	3862625..3864361 (-)	1737	WP_011052125.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 578 a.a.
• Molecular weight: 62517.63 Da
• Isoelectric Point: 5.4526

>NTDB_id=473175 HZS91_RS16640 WP_011052125.1 3862625..3864361(-) (pilB) [Xanthomonas citri pv. citri strain SN3-3]
MNSVVTANLVGITGIARRLVQDGAVEEAVARSAMDQASAAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLLDVSAFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSALG
DDEEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAANGVTSLAEINRVTKD

Nucleotide

• Length: 1737 bp

>NTDB_id=473175 HZS91_RS16640 WP_011052125.1 3862625..3864361(-) (pilB) [Xanthomonas citri pv. citri strain SN3-3]
ATGAATAGCGTCGTGACCGCAAATTTGGTTGGAATAACGGGCATCGCGCGTCGTCTGGTGCAAGACGGTGCGGTCGAAGA
AGCAGTTGCGCGATCTGCGATGGATCAGGCATCTGCGGCAAAGGTTCCTCTGCCGCAATGGTTTGCTGAGAAGAAGTTGG
TCACTGCTTCCCAGCTCGCCGCTGCGAATGCGGTCGAGTTCGGCATGCCACTGTTGGACGTGTCGGCGTTCGACGCCAGC
CAAAACGCGGTCAAGCTGGTAAGTGAGGAGCTACTTCAGAAGCACCAAGTGCTACCGCTGTTCAAGCGCGGCAACCGGCT
GTTCGTAGGGGTGAGCAATCCGACCCAGACCCGGGCGCTGGATGACATCAAGTTTCATACGAACTTGGTAGTCGAGCCCA
TCCTTGTGGATGAAGATCAGATCCGTCGCACCCTGGAGCAATGGCAGGCCAGCAATGCTGCGCTGGGCTCCGCGCTCGGT
GACGACGAGGAGGGCATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTTGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCTTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAACAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCGGCTTTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGTCTGATGAACATGGGCATCGCGCCCTACAACATCA
CCTCGTCGGTGACGCTGGTGATCGCGCAGCGGCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGATTCACGCCTGCCCAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
TGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTAATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGCTGATG
AAGGCTGCGAATGGGGTGACCAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-153)

• Type II/IV secretion system protein (T2SSE) (201-468)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	56.514	98.27	0.555
pilB	Acinetobacter baylyi ADP1	55.81	98.27	0.548
pilB	Legionella pneumophila strain ERS1305867	52.021	98.443	0.512
pilB	Vibrio cholerae strain A1552	49.306	99.654	0.491
pilF	Neisseria gonorrhoeae MS11	49.12	98.27	0.483
pilB	Vibrio parahaemolyticus RIMD 2210633	46.964	96.886	0.455
pilB	Vibrio campbellii strain DS40M4	45.518	98.443	0.448