NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	I6K20_RS15480	Genome accession	NZ_CP070048
Coordinates	3174915..3176039 (-)	Length	374 a.a.
NCBI ID	WP_000228540.1	Uniprot ID	-
Organism	Escherichia coli strain FDAARGOS_1290
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 3169915..3181039

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6K20_RS15450 (I6K20_15455)	yrdA	3171715..3172269 (+)	555	WP_001286217.1	gamma carbonic anhydrase family protein	-
I6K20_RS15455 (I6K20_15460)	yrdB	3172245..3172502 (-)	258	WP_001070562.1	DUF1488 domain-containing protein	-
I6K20_RS15460 (I6K20_15465)	aroE	3172499..3173317 (-)	819	WP_000451230.1	shikimate dehydrogenase	-
I6K20_RS15465 (I6K20_15470)	tsaC	3173322..3173894 (-)	573	WP_001310090.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
I6K20_RS15470 (I6K20_15475)	yrdD	3173899..3174441 (-)	543	WP_001129723.1	type I DNA topoisomerase	-
I6K20_RS15475 (I6K20_15480)	smg	3174470..3174943 (-)	474	WP_000460672.1	DUF494 family protein Smg	-
I6K20_RS15480 (I6K20_15485)	dprA	3174915..3176039 (-)	1125	WP_000228540.1	DNA-protecting protein DprA	Machinery gene
I6K20_RS15485 (I6K20_15490)	def	3176169..3176678 (+)	510	WP_000114984.1	peptide deformylase	-
I6K20_RS15490 (I6K20_15495)	fmt	3176693..3177640 (+)	948	WP_000004443.1	methionyl-tRNA formyltransferase	-
I6K20_RS15495 (I6K20_15500)	rsmB	3177686..3178975 (+)	1290	WP_001309796.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
I6K20_RS15500 (I6K20_15505)	trkA	3178997..3180373 (+)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
I6K20_RS15505 (I6K20_15510)	mscL	3180503..3180913 (+)	411	WP_000022442.1	large-conductance mechanosensitive channel protein MscL	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 41035.00 Da Isoelectric Point: 6.1815

                                >NTDB_id=472211 I6K20_RS15480 WP_000228540.1 3174915..3176039(-) (dprA) [Escherichia coli strain FDAARGOS_1290]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSYIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=472211 I6K20_RS15480 WP_000228540.1 3174915..3176039(-) (dprA) [Escherichia coli strain FDAARGOS_1290]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGTATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTTGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTCAGCATTGCTGTATTAGGGAATGGACTTAATACCATTCATCCGCGTCGCCATGCCCGACTGGCTACCAGTTTGCT

TGAACATGGTGGGGCTCTTGTCTCGGAATTTCCCCTCGATGTTCCTCCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGACGTGAAGTTTTTGCCTTGCCGGGTCCATTAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTAACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.509	98.663	0.479
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	48.71	82.888	0.404
dprA	Vibrio cholerae strain A1552	48.71	82.888	0.404
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	43.62	90.107	0.393
dprA	Legionella pneumophila strain ERS1305867	44.073	87.968	0.388

Detailed information NTG0472211