Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   I6L03_RS20980 Genome accession   NZ_CP069945
Coordinates   4396180..4397418 (-) Length   412 a.a.
NCBI ID   WP_071595741.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1373     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4391180..4402418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L03_RS20960 (I6L03_20960) dam 4391792..4392628 (-) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -
  I6L03_RS20965 (I6L03_20965) damX 4392735..4394021 (-) 1287 WP_000343172.1 cell division protein DamX -
  I6L03_RS20970 (I6L03_20970) aroB 4394113..4395201 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  I6L03_RS20975 (I6L03_20975) aroK 4395258..4395779 (-) 522 WP_000818618.1 shikimate kinase AroK -
  I6L03_RS20980 (I6L03_20980) pilQ 4396180..4397418 (-) 1239 WP_071595741.1 DNA uptake porin HofQ Machinery gene
  I6L03_RS20985 (I6L03_20985) hofP 4397330..4397734 (-) 405 WP_001264136.1 DNA utilization protein HofP -
  I6L03_RS20990 (I6L03_20990) hofO 4397724..4398164 (-) 441 WP_001055743.1 DNA utilization protein HofO -
  I6L03_RS20995 (I6L03_20995) hofN 4398148..4398687 (-) 540 WP_001069336.1 DNA utilization protein HofN -
  I6L03_RS21000 (I6L03_21000) hofM 4398687..4399466 (-) 780 WP_001296474.1 DNA utilization protein HofM -
  I6L03_RS21005 (I6L03_21005) mrcA 4399586..4402138 (+) 2553 WP_001296475.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44795.29 Da        Isoelectric Point: 5.9488

>NTDB_id=471303 I6L03_RS20980 WP_071595741.1 4396180..4397418(-) (pilQ) [Escherichia coli strain FDAARGOS_1373]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQVRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTTLGSDLSVATATTHIGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=471303 I6L03_RS20980 WP_071595741.1 4396180..4397418(-) (pilQ) [Escherichia coli strain FDAARGOS_1373]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAATATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGGTGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGTTGGCCGATGCGCAACAAGGTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTAGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

38.443

100

0.396
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  pilQ Pseudomonas aeruginosa PAK

34.633

100

0.367
  comE Glaesserella parasuis strain SC1401

35.545

100

0.364