Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H2789_RS01515 Genome accession   NZ_CP059549
Coordinates   323500..323949 (+) Length   149 a.a.
NCBI ID   WP_281836059.1    Uniprot ID   -
Organism   Acinetobacter calcoaceticus strain NCIMB13260     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 318500..328949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2789_RS01495 (H2789_01500) - 319884..320771 (+) 888 WP_005044026.1 metal-dependent hydrolase -
  H2789_RS01500 (H2789_01505) - 320900..321490 (+) 591 WP_003653797.1 LemA family protein -
  H2789_RS01505 (H2789_01510) - 321515..322594 (+) 1080 WP_209831809.1 TPM domain-containing protein -
  H2789_RS01510 (H2789_01515) - 322588..323148 (+) 561 WP_347019408.1 TPM domain-containing protein -
  H2789_RS01515 (H2789_01520) pilA 323500..323949 (+) 450 WP_281836059.1 pilin Machinery gene
  H2789_RS01520 (H2789_01525) - 324009..325319 (+) 1311 WP_347019409.1 O-antigen ligase family protein -
  H2789_RS01525 (H2789_01530) - 325425..327053 (+) 1629 WP_347019410.1 Wzy polymerase domain-containing protein -
  H2789_RS01530 (H2789_01535) bfr 327096..327560 (-) 465 WP_003653790.1 bacterioferritin -
  H2789_RS01535 (H2789_01540) - 327806..328000 (-) 195 WP_004639703.1 bacterioferritin-associated ferredoxin -
  H2789_RS01540 (H2789_01545) - 328220..328603 (-) 384 WP_002116996.1 RidA family protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15256.26 Da        Isoelectric Point: 7.7737

>NTDB_id=470484 H2789_RS01515 WP_281836059.1 323500..323949(+) (pilA) [Acinetobacter calcoaceticus strain NCIMB13260]
MNAQKGFTLIELMIVVAIIGILAAIAIPQYQNYIARSQASEAFTLADGLKATIATNLQSGTCFAEGKTTATAGTDDITGK
YGKATIVTSATAGSKCAISYKFNSSGVSNKLNASGADTIVADVGENGTLTKASSTTVPTEYLPQSFKAS

Nucleotide


Download         Length: 450 bp        

>NTDB_id=470484 H2789_RS01515 WP_281836059.1 323500..323949(+) (pilA) [Acinetobacter calcoaceticus strain NCIMB13260]
ATGAATGCACAAAAAGGTTTTACATTAATTGAACTTATGATCGTTGTTGCGATTATTGGGATTTTGGCTGCGATTGCGAT
TCCGCAATATCAAAACTATATCGCTCGTTCACAAGCTTCTGAGGCTTTTACTCTTGCAGATGGTTTAAAAGCAACAATTG
CGACAAACCTACAGTCTGGAACGTGTTTTGCAGAAGGAAAAACAACAGCAACAGCTGGTACGGATGATATTACTGGTAAG
TATGGTAAAGCTACAATCGTGACTAGTGCTACTGCTGGTAGTAAATGTGCTATCTCTTATAAATTTAATAGTTCAGGTGT
TTCTAATAAATTAAATGCATCTGGTGCAGATACAATTGTAGCTGATGTTGGTGAAAATGGTACTTTAACTAAAGCTTCTA
GCACTACGGTACCAACTGAATATTTACCGCAGTCTTTTAAAGCATCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter nosocomialis M2

67.114

100

0.671
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.456

98.658

0.389
  pilA Vibrio cholerae strain A1552

39.456

98.658

0.389
  pilA Vibrio cholerae C6706

39.456

98.658

0.389
  comP Acinetobacter baylyi ADP1

38.095

98.658

0.376