Detailed information NTG0470411

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H2784_RS13620
• Genome accession: NZ_CP059547
• Coordinates: 2936110..2937228 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain 10_3
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2931110..2942228

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H2784_RS13610 (H2784_13610)	-	2934226..2934918 (-)	693	WP_031962990.1	YggS family pyridoxal phosphate-dependent enzyme	-
H2784_RS13615 (H2784_13615)	pilT	2935045..2936082 (+)	1038	WP_223069599.1	type IV pilus twitching motility protein PilT	Machinery gene
H2784_RS13620 (H2784_13620)	pilU	2936110..2937228 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H2784_RS13625 (H2784_13625)	fur	2937298..2937735 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
H2784_RS13630 (H2784_13630)	-	2937847..2938245 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
H2784_RS13635 (H2784_13635)	-	2938300..2938623 (-)	324	WP_000442585.1	RnfH family protein	-
H2784_RS13640 (H2784_13640)	-	2938623..2939690 (-)	1068	WP_029776981.1	membrane protein	-
H2784_RS13645 (H2784_13645)	-	2939843..2940298 (-)	456	WP_000782976.1	bacteriohemerythrin	-
H2784_RS13650 (H2784_13650)	-	2940551..2941399 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=470411 H2784_RS13620 WP_000347036.1 2936110..2937228(+) (pilU) [Acinetobacter baumannii strain 10_3]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=470411 H2784_RS13620 WP_000347036.1 2936110..2937228(+) (pilU) [Acinetobacter baumannii strain 10_3]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGATTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392