Detailed information NTG0470336

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: H2785_RS01435
• Genome accession: NZ_CP059546
• Coordinates: 309279..310337 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain 10_4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 304279..315337

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H2785_RS01420 (H2785_01420)	-	305385..305717 (+)	333	WP_000169599.1	hypothetical protein	-
H2785_RS01425 (H2785_01425)	-	305720..306544 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
H2785_RS01430 (H2785_01430)	ponA	306562..309117 (-)	2556	WP_223069825.1	penicillin-binding protein 1A	-
H2785_RS01435 (H2785_01435)	pilM	309279..310337 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
H2785_RS01440 (H2785_01440)	pilN	310337..310978 (+)	642	WP_223069827.1	PilN domain-containing protein	Machinery gene
H2785_RS01445 (H2785_01445)	pilO	310975..311715 (+)	741	WP_002048088.1	type 4a pilus biogenesis protein PilO	Machinery gene
H2785_RS01450 (H2785_01450)	pilP	311726..312253 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
H2785_RS01455 (H2785_01455)	pilQ	312316..314481 (+)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
H2785_RS01460 (H2785_01460)	aroK	314493..315062 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=470336 H2785_RS01435 WP_002014066.1 309279..310337(+) (pilM) [Acinetobacter baumannii strain 10_4]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=470336 H2785_RS01435 WP_002014066.1 309279..310337(+) (pilM) [Acinetobacter baumannii strain 10_4]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCATTCTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423