NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	JSQ74_RS12245	Genome accession	NZ_CP069710
Coordinates	2538692..2539816 (-)	Length	374 a.a.
NCBI ID	WP_000228535.1	Uniprot ID	B7LHY7
Organism	Escherichia coli strain EA2
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 2533692..2544816

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JSQ74_RS12215 (JSQ74_12215)	yrdA	2535492..2536046 (+)	555	WP_001286216.1	gamma carbonic anhydrase family protein	-
JSQ74_RS12220 (JSQ74_12220)	yrdB	2536022..2536288 (-)	267	WP_204084414.1	DUF1488 domain-containing protein	-
JSQ74_RS12225 (JSQ74_12225)	aroE	2536279..2537094 (-)	816	Protein_2416	shikimate dehydrogenase	-
JSQ74_RS12230 (JSQ74_12230)	tsaC	2537099..2537671 (-)	573	WP_001297709.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
JSQ74_RS12235 (JSQ74_12235)	yrdD	2537676..2538218 (-)	543	WP_001129723.1	type I DNA topoisomerase	-
JSQ74_RS12240 (JSQ74_12240)	smg	2538247..2538720 (-)	474	WP_000460672.1	DUF494 family protein Smg	-
JSQ74_RS12245 (JSQ74_12245)	dprA	2538692..2539816 (-)	1125	WP_000228535.1	DNA-protecting protein DprA	Machinery gene
JSQ74_RS12250 (JSQ74_12250)	def	2539946..2540455 (+)	510	WP_000114984.1	peptide deformylase	-
JSQ74_RS12255 (JSQ74_12255)	fmt	2540470..2541417 (+)	948	WP_000004454.1	methionyl-tRNA formyltransferase	-
JSQ74_RS12260 (JSQ74_12260)	rsmB	2541463..2542752 (+)	1290	WP_001295160.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
JSQ74_RS12265 (JSQ74_12265)	trkA	2542774..2544150 (+)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
JSQ74_RS12270 (JSQ74_12270)	mscL	2544280..2544690 (+)	411	WP_000022442.1	large-conductance mechanosensitive channel protein MscL	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40954.92 Da Isoelectric Point: 6.3122

                                >NTDB_id=470019 JSQ74_RS12245 WP_000228535.1 2538692..2539816(-) (dprA) [Escherichia coli strain EA2]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLVTTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=470019 JSQ74_RS12245 WP_000228535.1 2538692..2539816(-) (dprA) [Escherichia coli strain EA2]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCAAATTGATGCGGTTGGATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGTGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGGTTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCTGCCTTACAGGTAAAT

GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCCCGACTGGCTGCCAGTCTGCT

TGAACATGGCGGAGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	B7LHY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.509	98.663	0.479
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	44.865	98.93	0.444
dprA	Vibrio cholerae strain A1552	44.865	98.93	0.444
dprA	Haemophilus influenzae Rd KW20	45.401	90.107	0.409
dprA	Glaesserella parasuis strain SC1401	44.838	90.642	0.406
dprA	Legionella pneumophila strain ERS1305867	44.545	88.235	0.393

Detailed information NTG0470019