Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   FOLKNPGA_RS05725 Genome accession   NZ_CP059400
Coordinates   1312669..1313268 (+) Length   199 a.a.
NCBI ID   WP_182352089.1    Uniprot ID   -
Organism   Legionella sp. PC1000     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1307669..1318268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOLKNPGA_RS05710 (FOLKNPGA_01171) - 1309645..1310781 (-) 1137 WP_182352086.1 hypothetical protein -
  FOLKNPGA_RS05715 (FOLKNPGA_01172) pilM 1311026..1312090 (+) 1065 WP_182352087.1 pilus assembly protein PilM Machinery gene
  FOLKNPGA_RS05720 (FOLKNPGA_01173) pilN 1312097..1312672 (+) 576 WP_182352088.1 PilN domain-containing protein Machinery gene
  FOLKNPGA_RS05725 (FOLKNPGA_01174) pilO 1312669..1313268 (+) 600 WP_182352089.1 type 4a pilus biogenesis protein PilO Machinery gene
  FOLKNPGA_RS05730 (FOLKNPGA_01175) pilP 1313265..1313783 (+) 519 WP_182352090.1 pilus assembly protein PilP Machinery gene
  FOLKNPGA_RS05735 (FOLKNPGA_01176) pilQ 1313792..1315897 (+) 2106 WP_182352091.1 type IV pilus secretin PilQ Machinery gene
  FOLKNPGA_RS05740 (FOLKNPGA_01177) aroK 1316428..1316955 (+) 528 WP_010653139.1 shikimate kinase AroK -
  FOLKNPGA_RS05745 (FOLKNPGA_01178) aroB 1316942..1318054 (+) 1113 WP_182352092.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22916.86 Da        Isoelectric Point: 6.4857

>NTDB_id=469372 FOLKNPGA_RS05725 WP_182352089.1 1312669..1313268(+) (pilO) [Legionella sp. PC1000]
MNSLSLNELTLENVGQWPVVVKSCVLMGVSILIIALGYWLIIQDNFTQFNKLKGEEATLKTDFENKQRQLFNLPAYRMQL
QLMMERFALMLKQLPEKNQMPGLLEDISKTGVASGLKFELFAPQPEVVHDFYIERPIKISVVGTYFQLAMFISRVAEMDR
IVTLHDFSIEGVSSKDKKVVSEDELVMNITAKIYRYRTQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=469372 FOLKNPGA_RS05725 WP_182352089.1 1312669..1313268(+) (pilO) [Legionella sp. PC1000]
ATGAATAGCCTTAGCCTGAATGAATTAACTCTGGAAAATGTTGGGCAATGGCCGGTAGTGGTTAAAAGTTGTGTTCTTAT
GGGAGTGAGTATTTTAATTATTGCTCTGGGTTATTGGCTAATCATACAAGACAACTTCACTCAGTTTAATAAACTAAAAG
GGGAAGAGGCTACTTTAAAAACAGATTTTGAAAACAAGCAACGCCAATTATTTAATTTGCCAGCCTATCGCATGCAGTTG
CAACTTATGATGGAGCGTTTTGCATTAATGCTCAAACAACTTCCTGAAAAAAATCAGATGCCTGGTTTATTGGAAGATAT
TTCTAAAACAGGAGTGGCATCCGGCTTGAAGTTTGAATTATTTGCGCCACAACCTGAGGTAGTACATGATTTTTATATTG
AGCGACCAATAAAAATTTCAGTAGTAGGAACTTATTTTCAATTGGCAATGTTTATAAGTCGGGTCGCTGAGATGGATCGT
ATTGTAACCTTGCATGACTTCAGTATTGAAGGGGTGTCCTCCAAAGATAAAAAAGTAGTTTCGGAGGATGAATTGGTGAT
GAACATCACAGCTAAAATATATCGATATCGCACACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(50-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

71.859

100

0.719
  comO Acinetobacter baylyi ADP1

40.314

95.98

0.387
  pilO Acinetobacter baumannii D1279779

38.86

96.985

0.377