Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H1D00_RS07035 Genome accession   NZ_CP059330
Coordinates   1414482..1415885 (-) Length   467 a.a.
NCBI ID   WP_240020920.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0722     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1409482..1420885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D00_RS07005 (H1D00_07060) lptE 1410249..1410737 (+) 489 WP_240020919.1 LPS assembly lipoprotein LptE -
  H1D00_RS07010 (H1D00_07065) holA 1410780..1411805 (+) 1026 WP_240021840.1 DNA polymerase III subunit delta -
  H1D00_RS07015 (H1D00_07070) yacG 1411806..1411991 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  H1D00_RS07020 (H1D00_07075) coaE 1411973..1412608 (-) 636 WP_240018838.1 dephospho-CoA kinase -
  H1D00_RS12900 - 1412822..1413028 (-) 207 Protein_1374 peptidase A24 -
  H1D00_RS07025 - 1413099..1413302 (-) 204 WP_240018335.1 hypothetical protein -
  H1D00_RS07030 (H1D00_07085) - 1413305..1414498 (-) 1194 Protein_1376 type II secretion system F family protein -
  H1D00_RS07035 (H1D00_07090) pilB 1414482..1415885 (-) 1404 WP_240020920.1 GspE/PulE family protein Machinery gene
  H1D00_RS07040 (H1D00_07095) pilA 1415906..1416313 (-) 408 WP_006251342.1 pilin Machinery gene
  H1D00_RS07045 (H1D00_07100) radA 1416565..1417944 (+) 1380 WP_240018337.1 DNA repair protein RadA -
  H1D00_RS07050 (H1D00_07105) - 1417999..1418229 (-) 231 WP_006251345.1 YgjV family protein -
  H1D00_RS07055 (H1D00_07110) - 1418349..1418843 (-) 495 WP_240018338.1 dihydrofolate reductase -
  H1D00_RS07060 (H1D00_07115) proB 1418955..1420046 (+) 1092 WP_240018339.1 glutamate 5-kinase -
  H1D00_RS07065 (H1D00_07120) - 1420046..1420810 (+) 765 WP_240020921.1 TSUP family transporter -

Sequence

Protein


Download         Length: 467 a.a.        Molecular weight: 52956.33 Da        Isoelectric Point: 5.3662

>NTDB_id=468747 H1D00_RS07035 WP_240020920.1 1414482..1415885(-) (pilB) [Mannheimia haemolytica strain 0722]
MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAVPLQETAEQLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSEEL
KFLLNALSPEQSSPQSEIYEENPLYPFSENTEQPDRNDPIIQILENLFKFCLQQNASDIHLEPQKERLLIRLRIDGVLHI
YKSLPSSLSSRLISRVKLLAKLDISETRLPQDGQFHFKTLLAETLDFRVSTLPTHFGEKVVLRLQKNKPTHFDFLDLGFN
PTQKANLLNALSQPQGLILVTGPTGSGKSITLYSALSHLNSSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRTF
LRQDPDIIMLGEIRDEESAEMALRAAQTGHLVLSTLHTNDAPSAVERLLQLGIKEYELKNSLLLVIAQRLLRRVCVKCGG
AGCEHCYQGYKGRIGVYQLLNRSAXNFEKNTACLDFKTLADSAKEKLTEGKTNQAEILRVLGNDENL

Nucleotide


Download         Length: 1404 bp        

>NTDB_id=468747 H1D00_RS07035 WP_240020920.1 1414482..1415885(-) (pilB) [Mannheimia haemolytica strain 0722]
ATGGAGTATTCGGTAACCGACTTAAACAGCCAAAGATTCATCGAGATTTCAGCAGAACAATGGCAGAAAAATTGTAATGA
AAAGCAGATTTTGTTGCGTTACTTAGCCGTTCCACTGCAAGAAACAGCAGAACAGCTTTGGCTGGGAATTGATGATATGC
AAAATCTTAATGCCTGCGAGATTTTTGCATTTATCTACCACAAACAGATTGAGCCTGTTTTGATTTCCTCCGAAGAATTA
AAATTTTTGCTCAATGCCCTTTCGCCTGAACAGAGTAGCCCGCAGAGTGAAATTTATGAAGAAAATCCGCTTTATCCTTT
TTCCGAGAATACCGAGCAGCCGGATAGAAATGATCCGATCATCCAAATTTTAGAGAATTTGTTTAAGTTCTGTTTGCAAC
AGAACGCTTCCGATATCCATCTTGAACCACAAAAAGAGAGATTGTTAATTCGGTTAAGAATTGATGGCGTGCTACATATT
TATAAAAGCCTGCCGAGTAGTTTATCCAGCCGTTTGATTTCACGGGTTAAATTATTGGCTAAGCTCGATATCAGCGAGAC
TCGTTTGCCACAAGACGGACAATTTCATTTCAAAACACTGTTAGCTGAAACGCTGGATTTCCGTGTTTCTACCTTGCCAA
CGCACTTCGGCGAAAAAGTGGTGCTACGGCTACAAAAAAATAAACCAACCCATTTTGATTTTTTAGATTTAGGCTTTAAC
CCAACTCAGAAAGCCAATTTGCTGAATGCTTTGAGTCAGCCTCAAGGATTGATTTTAGTAACCGGCCCGACAGGAAGCGG
TAAAAGCATCACGCTTTACAGTGCATTAAGCCATTTAAACAGCAGTGAAAAGCATATTTTGACTGCCGAAGACCCGATTG
AAATTGAGATTGAGGGAATTATCCAAACCCAAGTGAATCGAGGGATTAACTTAGATTTCAGCCAACTGTTAAGAACCTTT
CTACGCCAAGATCCGGACATTATTATGCTCGGCGAAATTCGTGATGAAGAGAGTGCAGAAATGGCACTACGGGCGGCACA
AACCGGGCATTTGGTGCTTTCGACTTTGCACACCAATGATGCCCCTTCAGCGGTAGAACGGTTGCTACAGCTCGGCATAA
AAGAGTATGAACTTAAAAATTCATTGCTACTCGTGATTGCTCAACGCCTGTTACGCCGAGTGTGTGTGAAATGCGGTGGG
GCAGGCTGTGAGCATTGTTATCAAGGGTATAAAGGGCGAATTGGGGTATATCAACTCTTAAACCGATCTGCANAAAATTT
TGAAAAAAACACCGCTTGTTTGGATTTTAAAACCCTTGCCGACAGTGCAAAAGAAAAATTGACGGAAGGAAAAACCAATC
AGGCGGAAATTTTAAGGGTGCTAGGCAATGACGAAAATCTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(132-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.234

98.715

0.713
  pilB Haemophilus influenzae Rd KW20

56.71

98.929

0.561
  pilB Haemophilus influenzae 86-028NP

55.94

99.143

0.555
  pilB Vibrio campbellii strain DS40M4

40.748

100

0.42
  pilB Vibrio cholerae strain A1552

40.249

100

0.415
  pilB Vibrio parahaemolyticus RIMD 2210633

38.755

100

0.413
  pilB Legionella pneumophila strain ERS1305867

39.344

100

0.411
  pilB Acinetobacter baylyi ADP1

35.928

100

0.385
  pilB Acinetobacter baumannii D1279779

36.401

100

0.381