NTDB

Overview

Name	comE	Type	Machinery gene
Locus tag	I6J95_RS01170	Genome accession	NZ_CP069583
Coordinates	267336..268574 (+)	Length	412 a.a.
NCBI ID	WP_000816005.1	Uniprot ID	B7UK96
Organism	Escherichia coli strain FDAARGOS_1265
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 262336..273574

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6J95_RS01145 (I6J95_01145)	mrcA	262616..265168 (-)	2553	WP_001296475.1	peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA	-
I6J95_RS01150 (I6J95_01150)	hofM	265288..266067 (+)	780	WP_001296474.1	DNA utilization protein HofM	-
I6J95_RS01155 (I6J95_01155)	hofN	266067..266606 (+)	540	WP_001069338.1	DNA utilization protein HofN	-
I6J95_RS01160 (I6J95_01160)	hofO	266590..267030 (+)	441	WP_001055748.1	DNA utilization protein HofO	-
I6J95_RS01165 (I6J95_01165)	hofP	267020..267424 (+)	405	WP_001264137.1	DNA utilization protein HofP	-
I6J95_RS01170 (I6J95_01170)	comE	267336..268574 (+)	1239	WP_000816005.1	DNA uptake porin HofQ	Machinery gene
I6J95_RS01175 (I6J95_01175)	aroK	268975..269496 (+)	522	WP_000818618.1	shikimate kinase AroK	-
I6J95_RS01180 (I6J95_01180)	aroB	269553..270641 (+)	1089	WP_000439857.1	3-dehydroquinate synthase	-
I6J95_RS01185 (I6J95_01185)	damX	270733..272019 (+)	1287	WP_000343172.1	cell division protein DamX	-
I6J95_RS01190 (I6J95_01190)	dam	272126..272962 (+)	837	WP_000742141.1	adenine-specific DNA-methyltransferase	-

Sequence

Protein

Download Length: 412 a.a. Molecular weight: 44811.29 Da Isoelectric Point: 5.9488

                                >NTDB_id=468457 I6J95_RS01170 WP_000816005.1 267336..268574(+) (comE) [Escherichia coli strain FDAARGOS_1265]

MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL

ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN

KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHIGFNIGRIN

GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL

HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL

VVFITPRLVSSE

Nucleotide

Download Length: 1239 bp

                                >NTDB_id=468457 I6J95_RS01170 WP_000816005.1 267336..268574(+) (comE) [Escherichia coli strain FDAARGOS_1265]

ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT

GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG

TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG

ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA

GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA

AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC

AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT

TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG

GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC

GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC

CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT

CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA

CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA

AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA

GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA

GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	B7UK96

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comE	Haemophilus influenzae 86-028NP	37.3	100	0.396
pilQ	Vibrio campbellii strain DS40M4	38.48	100	0.393
comE	Haemophilus influenzae Rd KW20	37.156	100	0.393
pilQ	Vibrio cholerae strain A1552	37.772	100	0.379
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	37.772	100	0.379
pilQ	Pseudomonas aeruginosa PAK	34.633	100	0.367
comE	Glaesserella parasuis strain SC1401	35.714	100	0.364

Detailed information NTG0468457