Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   I6J97_RS01470 Genome accession   NZ_CP069522
Coordinates   309085..310323 (+) Length   412 a.a.
NCBI ID   WP_000816011.1    Uniprot ID   A0A0H2Z3C0
Organism   Escherichia coli strain FDAARGOS_1267     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 304085..315323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J97_RS01445 (I6J97_01445) mrcA 304365..306917 (-) 2553 WP_001296475.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  I6J97_RS01450 (I6J97_01450) hofM 307037..307816 (+) 780 WP_001350443.1 DNA utilization protein HofM -
  I6J97_RS01455 (I6J97_01455) hofN 307816..308355 (+) 540 WP_001069334.1 DNA utilization protein HofN -
  I6J97_RS01460 (I6J97_01460) hofO 308339..308779 (+) 441 WP_001055745.1 DNA utilization protein HofO -
  I6J97_RS01465 (I6J97_01465) hofP 308769..309173 (+) 405 WP_001264138.1 DNA utilization protein HofP -
  I6J97_RS01470 (I6J97_01470) comE 309085..310323 (+) 1239 WP_000816011.1 DNA uptake porin HofQ Machinery gene
  I6J97_RS01475 (I6J97_01475) aroK 310724..311245 (+) 522 WP_001518996.1 shikimate kinase AroK -
  I6J97_RS01480 (I6J97_01480) aroB 311302..312390 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  I6J97_RS01485 (I6J97_01485) damX 312482..313768 (+) 1287 WP_000343176.1 cell division protein DamX -
  I6J97_RS01490 (I6J97_01490) dam 313875..314711 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44839.34 Da        Isoelectric Point: 5.9488

>NTDB_id=467746 I6J97_RS01470 WP_000816011.1 309085..310323(+) (comE) [Escherichia coli strain FDAARGOS_1267]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQVLAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHIGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=467746 I6J97_RS01470 WP_000816011.1 309085..310323(+) (comE) [Escherichia coli strain FDAARGOS_1267]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTTATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGTGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG
ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTCTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3C0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.3

100

0.396
  pilQ Vibrio campbellii strain DS40M4

38.48

100

0.393
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  pilQ Pseudomonas aeruginosa PAK

34.633

100

0.367
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364