Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HZS52_RS17695 Genome accession   NZ_CP059082
Coordinates   3816630..3818378 (+) Length   582 a.a.
NCBI ID   WP_190373267.1    Uniprot ID   -
Organism   Vreelandella titanicae strain SOB56     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3811630..3823378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZS52_RS17660 (HZS52_17660) - 3811885..3812814 (+) 930 WP_190373263.1 dihydrodipicolinate synthase family protein -
  HZS52_RS17665 (HZS52_17665) - 3812807..3813820 (+) 1014 WP_190373264.1 D-glycerate dehydrogenase -
  HZS52_RS24485 - 3813977..3814078 (+) 102 WP_223828144.1 IS66 family transposase zinc-finger binding domain-containing protein -
  HZS52_RS17680 (HZS52_17680) - 3814431..3815252 (-) 822 WP_190373265.1 inositol monophosphatase family protein -
  HZS52_RS17685 (HZS52_17685) - 3815347..3815781 (-) 435 WP_027959308.1 anti-virulence regulator CigR family protein -
  HZS52_RS17690 (HZS52_17690) - 3815894..3816388 (-) 495 WP_190373266.1 pilin -
  HZS52_RS17695 (HZS52_17695) pilB 3816630..3818378 (+) 1749 WP_190373267.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HZS52_RS17700 (HZS52_17700) pilC 3818382..3819614 (+) 1233 WP_022523592.1 type II secretion system F family protein Machinery gene
  HZS52_RS17705 (HZS52_17705) pilD 3819807..3820652 (+) 846 WP_022523593.1 A24 family peptidase Machinery gene
  HZS52_RS17710 (HZS52_17710) coaE 3820721..3821323 (+) 603 WP_190373268.1 dephospho-CoA kinase -
  HZS52_RS17715 (HZS52_17715) yacG 3821359..3821592 (+) 234 WP_022523595.1 DNA gyrase inhibitor YacG -
  HZS52_RS17720 (HZS52_17720) - 3821632..3822519 (+) 888 WP_022523596.1 hypothetical protein -
  HZS52_RS17725 (HZS52_17725) - 3822543..3822902 (-) 360 WP_035537408.1 hypothetical protein -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 64018.73 Da        Isoelectric Point: 6.9693

>NTDB_id=467158 HZS52_RS17695 WP_190373267.1 3816630..3818378(+) (pilB) [Vreelandella titanicae strain SOB56]
MSQPSLESTLSHASAHGDLRGIAQRLVKHGLLTNAQAATAETTASDLEISLLQHVIESGLVDPNKAILAAGWEYGLPVIN
LEAVRLTSLPPAADYPVKVLQSLNVLPLARHGHRLTVAVPYPATLTRLDELQFATGLSIEGILAPVDQITVVLNSYLAHN
ERGMLDELDDVVSSLSIMQNNGGNDVPLEESSQNSEDAPIVKFVNKILLDAIRRGASDIHFEPYEALYRVRFRVDGMLIE
VARPPFAMRNRIAARLKIMARLDISERRLPQDGSIKLKLSRTRSLDFRVNSLPTVYGEKLVLRILDPTAMQLGIEQLGFT
DEQRRHYEYALKQPQGMILVTGPTGSGKTVSLYTGINILNKVERNICTAEDPVEIKVAGINQVNVLPKIGLDFASALRAF
LRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGVSSFNIASAVSLIVAQRLARKLCTHCKE
PIDIPHEALRKEGFSSEEIHQATIYKPLGCKHCTHGYKGRIGIYEVVPITEAMRQLIMRDANALDIDQQVRKEGYPSLHR
SGLLKVMQGITSLEEVNRVSKE

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=467158 HZS52_RS17695 WP_190373267.1 3816630..3818378(+) (pilB) [Vreelandella titanicae strain SOB56]
ATGAGCCAGCCCTCCTTAGAGTCGACTTTAAGCCACGCCTCCGCACATGGCGACCTGCGCGGCATTGCCCAGCGCTTGGT
CAAGCACGGCTTGCTGACCAACGCTCAGGCCGCAACAGCGGAAACAACCGCGTCGGACTTGGAAATATCGTTACTTCAGC
ATGTGATCGAAAGCGGCCTGGTCGACCCTAACAAAGCCATACTGGCGGCTGGTTGGGAGTATGGGCTTCCAGTCATTAAC
CTGGAGGCGGTGCGGCTTACCTCACTGCCCCCCGCTGCAGACTATCCTGTCAAAGTACTGCAGAGCCTTAACGTTTTGCC
ATTAGCGCGCCACGGTCATCGCCTCACGGTGGCGGTGCCCTACCCCGCTACATTGACGCGACTGGATGAATTGCAGTTTG
CAACAGGCCTGAGCATTGAAGGTATCCTCGCGCCCGTTGATCAAATCACTGTCGTTCTCAATAGCTACCTTGCTCACAAC
GAGCGCGGCATGCTGGATGAGTTGGACGATGTGGTCAGCTCGCTGAGCATTATGCAGAACAACGGCGGTAACGATGTACC
GCTGGAAGAGTCTTCCCAAAATAGCGAAGATGCACCCATCGTTAAATTTGTGAACAAAATTCTGCTAGATGCTATCCGCC
GAGGCGCCTCCGATATCCATTTCGAACCTTACGAAGCGCTTTACCGAGTACGCTTTCGGGTTGATGGAATGCTCATAGAA
GTGGCACGGCCACCATTCGCCATGCGCAATCGTATCGCGGCCCGGCTAAAAATCATGGCCCGCCTTGATATTTCCGAGCG
CCGCCTGCCTCAGGATGGCTCGATAAAACTCAAGCTTTCGCGCACCCGCTCCCTCGATTTCCGGGTTAATTCGCTACCTA
CTGTCTATGGTGAAAAGTTGGTGCTGCGAATATTAGACCCTACCGCAATGCAGCTGGGTATTGAGCAACTTGGATTTACC
GACGAACAACGGCGCCATTATGAGTACGCCTTAAAACAACCCCAAGGCATGATTCTAGTAACCGGCCCTACCGGTAGTGG
TAAAACCGTTTCGCTGTATACCGGCATTAATATTCTTAATAAAGTGGAACGTAATATCTGCACCGCCGAAGACCCGGTTG
AAATCAAAGTCGCGGGTATCAATCAGGTAAATGTATTGCCTAAGATCGGCCTCGATTTTGCCAGTGCGCTAAGGGCGTTT
CTACGCCAAGACCCGGACGTCGTCATGGTAGGCGAGATTCGTGACCTGGAAACCGCCGAAATTGCCGTTAAAGCTGCACA
GACAGGCCACTTGGTACTCTCCACGGTGCATACCAACTCGGCAGCTGAGACACTGACTCGACTGGCTAATATGGGAGTCT
CTTCGTTTAATATCGCCAGCGCCGTTAGCTTGATTGTTGCCCAACGGCTGGCTCGTAAACTATGCACACACTGCAAAGAG
CCCATTGACATTCCCCACGAGGCGCTGCGCAAAGAAGGCTTTAGTAGCGAAGAAATCCATCAGGCAACGATCTACAAACC
GTTAGGTTGCAAGCACTGTACTCACGGTTATAAAGGTCGAATTGGCATTTATGAGGTAGTGCCGATCACCGAAGCGATGC
GTCAACTAATTATGCGTGATGCCAACGCCTTAGATATTGATCAACAGGTGCGCAAGGAGGGCTATCCAAGCCTTCACCGC
AGTGGGTTACTGAAAGTCATGCAGGGTATTACCAGCCTGGAAGAGGTTAACCGAGTGAGCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-160)

Type II/IV secretion system protein (T2SSE)

(206-473)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50.707

97.251

0.493
  pilB Acinetobacter baumannii D1279779

50.796

97.079

0.493
  pilB Acinetobacter baylyi ADP1

49.912

97.766

0.488
  pilB Vibrio campbellii strain DS40M4

48.789

99.313

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

47.945

100

0.481
  pilB Vibrio cholerae strain A1552

52.371

83.333

0.436
  pilF Neisseria gonorrhoeae MS11

49.688

82.646

0.411