Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   I6J85_RS03480 Genome accession   NZ_CP069447
Coordinates   754544..755782 (+) Length   412 a.a.
NCBI ID   WP_000815998.1    Uniprot ID   A0A0H2VBI8
Organism   Escherichia coli strain FDAARGOS_1255     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 749544..760782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J85_RS03455 (I6J85_03455) mrcA 749824..752376 (-) 2553 WP_001443173.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  I6J85_RS03460 (I6J85_03460) hofM 752496..753275 (+) 780 WP_001296474.1 DNA utilization protein HofM -
  I6J85_RS03465 (I6J85_03465) hofN 753275..753814 (+) 540 WP_001069336.1 DNA utilization protein HofN -
  I6J85_RS03470 (I6J85_03470) hofO 753798..754238 (+) 441 WP_001055743.1 DNA utilization protein HofO -
  I6J85_RS03475 (I6J85_03475) hofP 754228..754632 (+) 405 WP_001264136.1 DNA utilization protein HofP -
  I6J85_RS03480 (I6J85_03480) comE 754544..755782 (+) 1239 WP_000815998.1 DNA uptake porin HofQ Machinery gene
  I6J85_RS03485 (I6J85_03485) aroK 756183..756704 (+) 522 WP_000818618.1 shikimate kinase AroK -
  I6J85_RS03490 (I6J85_03490) aroB 756761..757849 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  I6J85_RS03495 (I6J85_03495) damX 757941..759227 (+) 1287 WP_000343172.1 cell division protein DamX -
  I6J85_RS03500 (I6J85_03500) dam 759334..760170 (+) 837 WP_042091857.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44781.26 Da        Isoelectric Point: 5.9488

>NTDB_id=466901 I6J85_RS03480 WP_000815998.1 754544..755782(+) (comE) [Escherichia coli strain FDAARGOS_1255]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHIGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=466901 I6J85_RS03480 WP_000815998.1 754544..755782(+) (comE) [Escherichia coli strain FDAARGOS_1255]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAATATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTCTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VBI8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.3

100

0.396
  pilQ Vibrio campbellii strain DS40M4

38.48

100

0.393
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  pilQ Vibrio cholerae strain A1552

38.015

100

0.381
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.015

100

0.381
  pilQ Pseudomonas aeruginosa PAK

34.633

100

0.367
  comE Glaesserella parasuis strain SC1401

35.476

100

0.362