Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GZH31_RS13515 Genome accession   NZ_CP058972
Coordinates   2992944..2994620 (+) Length   558 a.a.
NCBI ID   WP_118844625.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. JSTW     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2987944..2999620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZH31_RS13490 (GZH31_13490) - 2988060..2988503 (+) 444 WP_180311090.1 pilin -
  GZH31_RS13495 (GZH31_13495) - 2988550..2989845 (+) 1296 WP_180311091.1 O-antigen ligase family protein -
  GZH31_RS13500 (GZH31_13500) pilC 2989865..2991094 (+) 1230 WP_180311092.1 type II secretion system F family protein Machinery gene
  GZH31_RS13505 (GZH31_13505) pilD 2991274..2992170 (+) 897 WP_180311093.1 A24 family peptidase Machinery gene
  GZH31_RS13510 (GZH31_13510) coaE 2992173..2992781 (+) 609 WP_180311094.1 dephospho-CoA kinase -
  GZH31_RS13515 (GZH31_13515) pilB 2992944..2994620 (+) 1677 WP_118844625.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GZH31_RS13520 (GZH31_13520) yacG 2994688..2994921 (+) 234 WP_036965896.1 DNA gyrase inhibitor YacG -
  GZH31_RS13525 (GZH31_13525) - 2994983..2995513 (-) 531 WP_051501715.1 Spy/CpxP family protein refolding chaperone -
  GZH31_RS13530 (GZH31_13530) - 2995685..2996374 (+) 690 WP_036965892.1 response regulator -
  GZH31_RS13535 (GZH31_13535) - 2996376..2997761 (+) 1386 WP_118844628.1 ATP-binding protein -
  GZH31_RS13540 (GZH31_13540) - 2997762..2998703 (+) 942 WP_036965889.1 alpha/beta fold hydrolase -
  GZH31_RS13545 (GZH31_13545) - 2998682..2999590 (-) 909 WP_074988960.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62244.50 Da        Isoelectric Point: 5.4641

>NTDB_id=466262 GZH31_RS13515 WP_118844625.1 2992944..2994620(+) (pilB) [Pseudoalteromonas sp. JSTW]
MNINSPLLRKFITLGRIDAETVKLKQREYNSTAELISKSCGIDAHELAEQCTDLFRVPYFDIKDFDVSLISDELVKEKLI
REHHILPLIKKGRKLYIAASDPTDYGAFENFEFSTGLSCEVVVVDYNQLENKIEQLLDAAGSLHLTDDEFKELGDLDTDK
PRDMPSNDEDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHRYRVRFRIDGILHEMASPPNSLASRLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINTKADMTFANALKAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPEKLPDEELIRQGFDPATLSKLTL
FSPKGCDSCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLKSGFNNLRLSGLKKAADGVTSLAEINRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=466262 GZH31_RS13515 WP_118844625.1 2992944..2994620(+) (pilB) [Pseudoalteromonas sp. JSTW]
ATGAATATTAACTCACCGTTACTGAGAAAATTTATCACATTAGGTCGCATTGATGCAGAGACCGTCAAATTAAAGCAACG
AGAATATAACTCCACAGCTGAGCTCATTTCTAAGAGCTGCGGTATAGACGCGCATGAATTAGCAGAGCAATGCACTGACT
TGTTCCGTGTTCCCTATTTTGATATCAAAGACTTCGATGTATCACTTATTTCTGATGAGTTAGTAAAAGAAAAGCTCATT
CGCGAGCATCATATCCTCCCGCTGATAAAGAAAGGCCGTAAGCTCTATATAGCGGCATCGGACCCTACTGATTACGGTGC
GTTTGAAAACTTTGAATTTAGTACAGGTCTGTCTTGTGAAGTTGTAGTGGTTGATTACAACCAGCTAGAAAATAAAATAG
AGCAGCTGCTCGATGCGGCCGGTAGCTTACATTTAACGGATGACGAATTTAAAGAGCTTGGCGATCTCGACACCGATAAA
CCACGCGATATGCCAAGTAATGATGAAGACAAAGACGATGCGCCTATTATTGTCTACATCAATAAGATTTTGATGGATGC
AATAAAAAAAGGGGCATCAGATTTACACTTCGAGCCATACGAGCATCGTTATCGAGTGCGCTTTCGGATTGATGGCATTT
TACACGAAATGGCAAGCCCACCGAATAGCCTAGCAAGTCGATTATCTGCGCGCATAAAAGTTATGTCACGCTTAGATATC
GCCGAAAAACGTAAACCACAAGATGGCCGCATTAAATTAAAAATTACTGAACGTAAAAGTATCGACTTTCGTGTCAGTAC
CCTGCCAACGCTGTGGGGCGAAAAAATTGTAATGCGTATTCTTGATTCATCAAGTGCTATGCTTGGCATCGATGTATTGG
GTTATGAGCCTGAACAGAAAAAGCTCTACATGGATGCGCTCGAACAGCCGCAAGGGATGATATTAGTGACAGGTCCTACA
GGCTCTGGTAAAACAGTATCTCTTTACACCGGCTTGAATATTTTGAACCAACCTGAGCGCAATATTAGTACTGCGGAAGA
TCCGGTTGAGATCAACCTTGAAGGCATTAACCAAGTACAAATTAACACAAAAGCAGATATGACGTTCGCCAACGCGTTAA
AAGCGTTCTTGCGTCAAGATCCCGATGTCGTCATGGTCGGTGAGATACGAGATCTAGAAACAGCTGAAATTTCCATTAAA
GCAGCGCAAACAGGCCACCTAGTCTTATCGACACTGCACACAAATTCAGCGCCAGAAACACTGACTCGATTGTTAAACAT
GGGCGTTCCGGCATACAACGTTGCAAGTTCAATTAGTCTTATCATTGCACAGCGACTGGCCAGACGTTTATGTCCGAAAT
GTAAAACCCCAGAAAAACTGCCCGATGAAGAACTTATTCGCCAAGGCTTTGACCCCGCAACATTGAGCAAACTCACGCTG
TTTTCTCCAAAAGGCTGTGATAGCTGTACCGATGGCTATAAGGGACGAGTAGGTATTTACGAAGTCATGCAAATTACCCC
TGAAATTGCGCAAATTATTATGCGTGGAGGTAATTCACTCGAAATTGCTGAGGTTTCTTTAAAATCAGGATTTAATAACT
TACGTCTATCGGGATTAAAAAAAGCGGCGGATGGTGTCACCTCGCTAGCTGAAATAAATCGGGTCACGAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.371

100

0.579
  pilB Acinetobacter baylyi ADP1

55.536

100

0.557
  pilB Legionella pneumophila strain ERS1305867

53.789

96.953

0.522
  pilB Vibrio cholerae strain A1552

54.023

93.548

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

52.247

95.699

0.5
  pilB Vibrio campbellii strain DS40M4

52.06

95.699

0.498
  pilF Neisseria gonorrhoeae MS11

49.423

93.19

0.461
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.772

93.369

0.362