Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HZU75_RS06915 Genome accession   NZ_CP058952
Coordinates   1495697..1496887 (-) Length   396 a.a.
NCBI ID   WP_180308403.1    Uniprot ID   A0A7D5V9A5
Organism   Chitinibacter fontanus strain STM-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1490697..1501887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZU75_RS06900 (HZU75_06900) - 1491386..1491829 (-) 444 WP_180308401.1 DciA family protein -
  HZU75_RS06905 (HZU75_06905) - 1491844..1492728 (+) 885 WP_180308402.1 M23 family metallopeptidase -
  HZU75_RS06910 (HZU75_06910) secA 1492848..1495589 (+) 2742 WP_228028223.1 preprotein translocase subunit SecA -
  HZU75_RS06915 (HZU75_06915) pilU 1495697..1496887 (-) 1191 WP_180308403.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HZU75_RS06920 (HZU75_06920) - 1497013..1497609 (+) 597 WP_180308404.1 TetR/AcrR family transcriptional regulator -
  HZU75_RS06925 (HZU75_06925) - 1497690..1498847 (+) 1158 WP_180308405.1 iron-containing alcohol dehydrogenase -
  HZU75_RS06930 (HZU75_06930) - 1498983..1499273 (+) 291 WP_180308406.1 hypothetical protein -
  HZU75_RS06935 (HZU75_06935) - 1499671..1500177 (-) 507 WP_180308407.1 hypothetical protein -
  HZU75_RS06940 (HZU75_06940) - 1500178..1500672 (-) 495 WP_180308408.1 SMI1/KNR4 family protein -
  HZU75_RS06945 (HZU75_06945) - 1500691..1501170 (-) 480 WP_180308409.1 DUF4265 domain-containing protein -

Sequence

Protein


Download         Length: 396 a.a.        Molecular weight: 44309.86 Da        Isoelectric Point: 6.2334

>NTDB_id=466133 HZU75_RS06915 WP_180308403.1 1495697..1496887(-) (pilU) [Chitinibacter fontanus strain STM-7]
MSPEQALKTMHDLLRMMIQHKASDLFISDDFPPAMKINGKMTPVGQQKLNAAATRALAYALMRPDQVAEFEGEFECNFAI
SPEGIGRFRVNVFMQQEKVGMVLRTITSKIPNFDEMGLPKVLKEVMMSKRGLVLLVGGTGSGKSTTMAAMIDYRNEHSHG
HIITIEDPVEYLHKNKNCLVTHREVGRDTKGWFSALKNTLRQAPDVILIGEIRDRETMEYAMNFAETGHLCMGTLHANSS
NQALERIVNFFPEERHPQLYMDLSLNMRGIISQRLVPTPDGKGRCAAIEILLNTPLVADLIFKGDVGGVKTAMQKSRELG
MQTFDMALFDLYEAGKISMEEALKNADSLNELRLNIKLNSQHHRKEEHSSGLDHLSLEVHEEPETESTDVEGNTAG

Nucleotide


Download         Length: 1191 bp        

>NTDB_id=466133 HZU75_RS06915 WP_180308403.1 1495697..1496887(-) (pilU) [Chitinibacter fontanus strain STM-7]
ATGAGCCCAGAACAAGCCCTAAAAACCATGCATGATTTGCTGCGCATGATGATTCAACACAAAGCGTCCGATTTATTTAT
CTCCGATGATTTTCCACCCGCAATGAAAATCAACGGGAAGATGACGCCGGTGGGGCAGCAAAAACTCAACGCAGCGGCAA
CTCGCGCCTTGGCTTATGCGCTGATGCGGCCCGATCAGGTGGCTGAGTTTGAGGGCGAATTTGAGTGCAATTTTGCGATT
AGCCCCGAAGGCATCGGTCGCTTTCGCGTGAATGTGTTTATGCAGCAGGAAAAAGTGGGCATGGTGTTGCGCACGATTAC
CAGCAAGATTCCAAACTTCGACGAGATGGGCCTGCCCAAAGTATTAAAAGAAGTGATGATGAGTAAGCGCGGGCTGGTGC
TGCTGGTCGGTGGTACCGGCTCGGGTAAATCGACGACGATGGCGGCGATGATTGATTACCGCAACGAGCACAGCCACGGC
CACATCATCACAATTGAAGATCCGGTTGAGTATCTGCATAAGAATAAAAATTGTCTGGTGACGCATCGCGAAGTAGGGCG
TGACACCAAGGGCTGGTTTAGCGCGCTAAAAAATACGCTGCGCCAAGCGCCCGATGTGATTTTGATTGGTGAGATTCGCG
ATCGTGAAACGATGGAATACGCGATGAACTTCGCCGAAACCGGCCACCTATGCATGGGCACGCTGCATGCCAATAGCTCT
AATCAGGCGCTCGAACGCATCGTTAATTTTTTCCCCGAAGAGCGCCATCCGCAGCTGTATATGGATTTATCGCTCAATAT
GCGCGGCATCATTTCGCAACGACTGGTGCCAACGCCCGATGGCAAAGGGCGCTGCGCGGCGATCGAGATTTTACTAAATA
CGCCCCTGGTGGCCGACTTAATTTTCAAAGGCGATGTTGGTGGCGTGAAAACCGCAATGCAAAAATCGCGCGAGCTGGGC
ATGCAAACCTTTGATATGGCGCTATTTGACTTGTATGAGGCGGGTAAAATCAGCATGGAAGAAGCGCTGAAAAATGCCGA
TTCACTCAATGAATTGCGGCTCAATATCAAGCTCAATAGCCAGCATCACCGCAAAGAGGAACACAGCAGCGGCTTGGATC
ATTTGTCACTGGAGGTGCACGAAGAGCCGGAAACGGAGTCGACCGATGTCGAGGGTAACACTGCTGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D5V9A5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.026

88.131

0.52
  pilU Acinetobacter baylyi ADP1

55.524

89.141

0.495
  pilU Vibrio cholerae strain A1552

52.958

89.646

0.475
  pilT Pseudomonas aeruginosa PAK

43.582

84.596

0.369
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.323

85.101

0.369