Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JHS95_RS02920 Genome accession   NZ_CP068627
Coordinates   595664..596089 (-) Length   141 a.a.
NCBI ID   WP_140311744.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain LH80     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 590664..601089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHS95_RS02900 (JHS95_02905) coaE 591178..591792 (-) 615 WP_005480887.1 dephospho-CoA kinase -
  JHS95_RS02905 (JHS95_02910) pilD 591793..592662 (-) 870 WP_237301937.1 prepilin peptidase Machinery gene
  JHS95_RS02910 (JHS95_02915) pilC 592727..593950 (-) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  JHS95_RS02915 (JHS95_02920) pilB 593974..595659 (-) 1686 WP_237301938.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JHS95_RS02920 (JHS95_02925) pilA 595664..596089 (-) 426 WP_140311744.1 type IV pilin protein Machinery gene
  JHS95_RS02925 (JHS95_02930) nadC 596351..597238 (-) 888 WP_140311742.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JHS95_RS02930 (JHS95_02935) ampD 597331..597882 (+) 552 WP_005462574.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JHS95_RS02935 (JHS95_02940) pdhR 598288..599055 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14722.75 Da        Isoelectric Point: 9.4846

>NTDB_id=460140 JHS95_RS02920 WP_140311744.1 595664..596089(-) (pilA) [Vibrio parahaemolyticus strain LH80]
MKNSKQKKQQGFTLIELMIVVAIIGVLSAVAIPAYKNYVKKSEAAAGMGTVRALLTNIDMYIQENGSFPTTGKLADLGAS
AGMNTLGTIALTQDGSVSEAGSVAFNFGNNATLNSTNITYTRSASGWSCNNTTGQTIKSCN

Nucleotide


Download         Length: 426 bp        

>NTDB_id=460140 JHS95_RS02920 WP_140311744.1 595664..596089(-) (pilA) [Vibrio parahaemolyticus strain LH80]
ATGAAAAACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTAATGATTGTCGTCGCGATTATTGGCGTATT
ATCAGCTGTGGCAATTCCCGCTTACAAAAACTATGTAAAAAAATCTGAAGCTGCTGCTGGAATGGGCACAGTACGTGCTC
TTTTAACTAATATTGATATGTACATTCAAGAAAATGGCTCATTCCCCACAACAGGTAAACTTGCAGATCTTGGAGCCTCT
GCAGGTATGAACACCCTTGGTACAATAGCATTGACTCAAGATGGAAGCGTCTCAGAAGCTGGAAGCGTTGCATTTAACTT
CGGCAATAACGCAACACTAAATAGTACAAATATCACATATACTCGAAGTGCCTCTGGCTGGTCTTGTAATAACACTACAG
GACAAACTATTAAAAGCTGTAATTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(41-132)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.371

100

0.475
  pilA Vibrio cholerae strain A1552

44.371

100

0.475
  pilA Vibrio cholerae C6706

44.371

100

0.475
  pilA Vibrio parahaemolyticus RIMD 2210633

50.376

94.326

0.475
  pilA Pseudomonas aeruginosa PAK

41.912

96.454

0.404