Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   SA190iCDA_RS00650 Genome accession   NZ_CP056087
Coordinates   138636..139700 (-) Length   354 a.a.
NCBI ID   WP_070885969.1    Uniprot ID   -
Organism   Pseudomonas argentinensis strain SA190     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 133636..144700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA190iCDA_RS00625 aroK 134181..134699 (-) 519 WP_027903890.1 shikimate kinase AroK -
  SA190iCDA_RS00630 pilQ 134703..136874 (-) 2172 WP_070885973.1 type IV pilus secretin PilQ Machinery gene
  SA190iCDA_RS00635 pilP 136929..137447 (-) 519 WP_070885972.1 type 4a pilus biogenesis lipoprotein PilP -
  SA190iCDA_RS00640 pilO 137444..138067 (-) 624 WP_070885971.1 type 4a pilus biogenesis protein PilO -
  SA190iCDA_RS00645 - 138064..138636 (-) 573 WP_070885970.1 PilN domain-containing protein -
  SA190iCDA_RS00650 pilM 138636..139700 (-) 1065 WP_070885969.1 pilus assembly protein PilM Machinery gene
  SA190iCDA_RS00655 - 139885..142320 (+) 2436 WP_419203826.1 penicillin-binding protein 1A -
  SA190iCDA_RS00660 - 142447..143715 (-) 1269 WP_070885967.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37965.26 Da        Isoelectric Point: 4.4260

>NTDB_id=458110 SA190iCDA_RS00650 WP_070885969.1 138636..139700(-) (pilM) [Pseudomonas argentinensis strain SA190]
MLGLFSKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVRSVSV
AVAGSAVITKTVEMEAGLSDDELENQLKLEADQYIPYPLEEVAIDFEVQGISPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYDLLAEQLGGGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
DEAGLAKKQGGLPDDYESEVLQPFKDALVQQVSRSLQFFFAAGQYNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=458110 SA190iCDA_RS00650 WP_070885969.1 138636..139700(-) (pilM) [Pseudomonas argentinensis strain SA190]
GTGCTAGGGCTCTTCAGTAAAAAAGCGAATACGCTTTTGGGGATCGATATAAGTTCGACCTCCGTCAAGCTCCTCGAATT
GAGTCGCTCAGGTAGCCGCTACAAGGTAGAGGCCTACGCAGTCGAGCCGCTCCCCGCCAATGCTGTGGTCGAAAAGAACA
TCGCCGAACTCGAAGGGGTCGGCCAGGCGTTGTCGCGTGTCCTGGCCAAGGCCAAGACCAGCGTGCGCTCGGTTTCCGTG
GCCGTTGCCGGCTCCGCGGTGATCACCAAGACCGTCGAGATGGAAGCGGGCCTGTCGGACGACGAGCTGGAGAACCAGCT
CAAGCTCGAAGCCGACCAGTACATTCCCTACCCCCTGGAAGAGGTGGCGATCGACTTCGAAGTTCAGGGCATTTCGCCGC
GCAACCCCGAGCGGGTCGAAGTGCTGCTTGCAGCCTGCCGCAAGGAAAACGTCGAGGTTCGCGAGGCCGCCCTGGCCCTT
GCCGGGCTGACCGCCAAGGTCGTCGATGTCGAAGCCTATGCCCTCGAGCGTGCCTACGACCTGCTGGCCGAACAGCTCGG
CGGTGGACACGACGAATTGACCGTGGCCGTCGTCGATATCGGCGCCACCATGACCACGCTCAGCGTGCTGCACAATGGCC
GCACCATCTATACCCGCGAGCAGCTGTTCGGTGGCCGCCAGCTGACCGAGGAAATCCAGCGTCGCTATGGCCTGTCCGTC
GATGAAGCGGGGCTTGCCAAGAAGCAGGGCGGCCTGCCCGACGACTACGAAAGCGAAGTGTTGCAGCCCTTCAAGGATGC
ATTGGTACAGCAGGTGTCGCGCTCTCTGCAGTTCTTCTTTGCCGCCGGCCAGTACAACGATGTGGATTACATCCTGCTGG
CCGGTGGAACCGCCTCCATCCCGGATCTCGATCGCCTCATTCAGCAGAAGATAGGCACCCAGACCCTGGTCGCCAACCCG
TTCGCGGACATGGCCCTGAGCGGCAAGGTCAATGCGGGGGCGCTGGCCAGTGATGCACCGGCACTGATGATTGCCTGCGG
CCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463