NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	JJB21_RS16395	Genome accession	NZ_CP067311
Coordinates	3395159..3396283 (+)	Length	374 a.a.
NCBI ID	WP_000228551.1	Uniprot ID	P30852
Organism	Escherichia coli strain 027_SK3
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 3390159..3401283

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JJB21_RS16370 (JJB21_16365)	mscL	3390285..3390695 (-)	411	WP_000022442.1	large-conductance mechanosensitive channel protein MscL	-
JJB21_RS16375 (JJB21_16370)	trkA	3390825..3392201 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
JJB21_RS16380 (JJB21_16375)	rsmB	3392223..3393512 (-)	1290	WP_000744778.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
JJB21_RS16385 (JJB21_16380)	fmt	3393558..3394505 (-)	948	WP_000004473.1	methionyl-tRNA formyltransferase	-
JJB21_RS16390 (JJB21_16385)	def	3394520..3395029 (-)	510	WP_000114984.1	peptide deformylase	-
JJB21_RS16395 (JJB21_16390)	dprA	3395159..3396283 (+)	1125	WP_000228551.1	DNA-protecting protein DprA	Machinery gene
JJB21_RS16400 (JJB21_16395)	smg	3396255..3396728 (+)	474	WP_000460680.1	DUF494 family protein Smg	-
JJB21_RS16405 (JJB21_16400)	yrdD	3396757..3397299 (+)	543	WP_001129722.1	type I DNA topoisomerase	-
JJB21_RS16410 (JJB21_16405)	tsaC	3397304..3397876 (+)	573	WP_001301412.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
JJB21_RS16415 (JJB21_16410)	aroE	3397881..3398699 (+)	819	WP_000451243.1	shikimate dehydrogenase	-
JJB21_RS16420 (JJB21_16415)	yrdB	3398696..3398953 (+)	258	WP_001070563.1	DUF1488 domain-containing protein	-
JJB21_RS16425 (JJB21_16420)	yrdA	3398929..3399483 (-)	555	WP_001286216.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40954.92 Da Isoelectric Point: 6.3122

                                >NTDB_id=457906 JJB21_RS16395 WP_000228551.1 3395159..3396283(+) (dprA) [Escherichia coli strain 027_SK3]

MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=457906 JJB21_RS16395 WP_000228551.1 3395159..3396283(+) (dprA) [Escherichia coli strain 027_SK3]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATCGCTCACTG

GGTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCACGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATTCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT

GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT

TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	P30852

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.238	98.663	0.476
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	49.355	82.888	0.409
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Haemophilus influenzae Rd KW20	45.104	90.107	0.406
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0457906