Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HV560_RS06635 Genome accession   NZ_CP055305
Coordinates   1371222..1371671 (+) Length   149 a.a.
NCBI ID   WP_176812458.1    Uniprot ID   -
Organism   Mannheimia pernigra strain 17CN0883     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1366222..1376671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV560_RS06610 (HV560_06610) - 1366799..1367563 (-) 765 WP_176812456.1 TSUP family transporter -
  HV560_RS06615 (HV560_06615) proB 1367563..1368654 (-) 1092 WP_159629587.1 glutamate 5-kinase -
  HV560_RS06620 (HV560_06620) - 1368768..1369262 (+) 495 WP_159629590.1 dihydrofolate reductase -
  HV560_RS06625 (HV560_06625) - 1369390..1369623 (+) 234 WP_159629593.1 YgjV family protein -
  HV560_RS06630 (HV560_06630) radA 1369662..1371041 (-) 1380 WP_176812457.1 DNA repair protein RadA Machinery gene
  HV560_RS06635 (HV560_06635) pilA 1371222..1371671 (+) 450 WP_176812458.1 prepilin peptidase-dependent pilin Machinery gene
  HV560_RS06640 (HV560_06640) pilB 1371693..1373093 (+) 1401 WP_176812459.1 GspE/PulE family protein Machinery gene
  HV560_RS06645 (HV560_06645) pilC 1373077..1374270 (+) 1194 WP_176812460.1 type II secretion system F family protein Machinery gene
  HV560_RS06650 (HV560_06650) - 1374273..1374929 (+) 657 WP_176812461.1 prepilin peptidase -
  HV560_RS06655 (HV560_06655) coaE 1374961..1375599 (+) 639 WP_176812462.1 dephospho-CoA kinase -
  HV560_RS06660 (HV560_06660) yacG 1375581..1375766 (+) 186 WP_159629615.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16043.32 Da        Isoelectric Point: 8.0859

>NTDB_id=457506 HV560_RS06635 WP_176812458.1 1371222..1371671(+) (pilA) [Mannheimia pernigra strain 17CN0883]
MQKIYLFRPLAKAFTLIELMIVIAIIAILATIAIPSYNAYTQKAALSELLRASASYKSDVELCIYNRNELDNCSGGTNGI
QANKADTSDTKYLKTITVNKGVITVTGKGSLDGYSYTLTPAFSNNTISWSASCTASNNDTSLFPAGFCN

Nucleotide


Download         Length: 450 bp        

>NTDB_id=457506 HV560_RS06635 WP_176812458.1 1371222..1371671(+) (pilA) [Mannheimia pernigra strain 17CN0883]
ATGCAAAAAATTTATCTTTTTCGACCGCTTGCAAAAGCGTTTACCTTGATTGAGCTAATGATCGTGATTGCAATTATTGC
CATTCTAGCCACTATTGCGATTCCATCTTACAACGCTTACACACAAAAAGCGGCTTTATCTGAATTATTGAGAGCATCGG
CTTCTTATAAATCAGATGTTGAGCTGTGTATTTATAACCGTAATGAGCTGGATAATTGTTCGGGCGGTACAAATGGTATT
CAAGCCAATAAAGCTGATACTAGCGACACCAAATATCTTAAGACGATTACGGTAAATAAAGGTGTGATTACCGTAACAGG
AAAAGGCTCGCTTGACGGCTATAGCTACACGCTAACACCAGCCTTTTCCAATAATACGATCAGTTGGAGTGCAAGTTGTA
CCGCCTCGAATAATGATACCAGCTTGTTCCCCGCAGGTTTTTGTAATTAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

69.333

100

0.698
  pilA Haemophilus influenzae 86-028NP

55.102

98.658

0.544
  pilA Haemophilus influenzae Rd KW20

52.349

100

0.523