Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   JJB17_RS16435 Genome accession   NZ_CP067245
Coordinates   3442257..3443495 (+) Length   412 a.a.
NCBI ID   WP_208789777.1    Uniprot ID   -
Organism   Escherichia coli strain OW1E2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3437257..3448495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJB17_RS16410 (JJB17_16405) mrcA 3437538..3440090 (-) 2553 WP_113078398.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  JJB17_RS16415 (JJB17_16410) hofM 3440210..3440989 (+) 780 WP_001295166.1 DNA utilization protein HofM -
  JJB17_RS16420 (JJB17_16415) hofN 3440989..3441528 (+) 540 WP_001586094.1 DNA utilization protein HofN -
  JJB17_RS16425 (JJB17_16420) hofO 3441512..3441937 (+) 426 Protein_3256 DNA utilization protein HofO -
  JJB17_RS16430 (JJB17_16425) hofP 3441941..3442345 (+) 405 WP_113078407.1 DNA utilization protein HofP -
  JJB17_RS16435 (JJB17_16430) comE 3442257..3443495 (+) 1239 WP_208789777.1 DNA uptake porin HofQ Machinery gene
  JJB17_RS16440 (JJB17_16435) aroK 3443896..3444417 (+) 522 WP_000818618.1 shikimate kinase AroK -
  JJB17_RS16445 (JJB17_16440) aroB 3444474..3445562 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  JJB17_RS16450 (JJB17_16445) damX 3445654..3446940 (+) 1287 WP_000343195.1 cell division protein DamX -
  JJB17_RS16455 (JJB17_16450) dam 3447047..3447883 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44710.19 Da        Isoelectric Point: 6.3183

>NTDB_id=457419 JJB17_RS16435 WP_208789777.1 3442257..3443495(+) (comE) [Escherichia coli strain OW1E2]
MKQWIAALLLMLIPGVQAAKSQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQTASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=457419 JJB17_RS16435 WP_208789777.1 3442257..3443495(+) (comE) [Escherichia coli strain OW1E2]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGTCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGACTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

37.767

100

0.386
  comE Haemophilus influenzae 86-028NP

39.043

96.359

0.376
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.288

100

0.374
  pilQ Vibrio cholerae strain A1552

37.288

100

0.374
  comE Glaesserella parasuis strain SC1401

36.19

100

0.369
  pilQ Pseudomonas aeruginosa PAK

34.091

100

0.364